FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51951
Sequences flagged as poor quality0
Sequence length301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA13882.6717483782795326No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA13112.523531789570942No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9291.7882235183153357No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9201.7708995014532924No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8641.6631056187561357No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8461.6284575850320493No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8121.5630112991087755No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7821.5052645762352985No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7311.4070951473503879No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7001.3474235337144618No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA6231.1992069450058709No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA6071.1684086928066832No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5241.008642759523397No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA5080.9778445073242094No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4900.9431964736001232No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4890.9412715828376739No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA4840.9316471290254279No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4580.8815999692017478No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4530.8719755153895016No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA4450.8565763892899079No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4420.8508017170025601No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4390.8450270447152124No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4330.8334777001405171No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA4270.8219283555658217No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4240.8161536832784739No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4030.7757309772670401No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3920.7545571788800985No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3840.7391580527805047No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3770.7256838174433601No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3640.7006602375315201No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA3430.6602375315200862No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3250.625589497796No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA3220.6198148255086524No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3180.6121152624588555No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA3160.608265480933957No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA3030.5832419010221169No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA3010.5793921194972186No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2890.5562934303478277No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2890.5562934303478277No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA2840.5466689765355817No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2700.5197205058612924No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2620.5043213797616986No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2610.5023964889992493No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2600.5004715982368001No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2560.49277203518700313No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2560.49277203518700313No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2540.4889222536621047No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2520.48507247213720617No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA2410.4638986737502647No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2410.4638986737502647No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2360.4542742199380185No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2310.4446497661257724No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2270.43695020307597543No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2260.43502531231352615No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2200.4234759677388308No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2200.4234759677388308No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2190.42155107697638156No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2100.40422706011433845No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2080.40037727858944006No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1860.35802968181555694No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1850.35610479105310777No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1830.35225500952820926No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1800.3464803372408616No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1770.34070566495351384No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1750.33685588342861544No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1730.33300610190371693No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1710.3291563203788185No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1660.31953186656657234No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1660.31953186656657234No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1620.31183230351677543No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1590.30605763122942775No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1570.30220784970452924No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1560.30028295894208No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1530.29450828665473233No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1520.2925833958922831No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1510.2906585051298339No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1450.2791091605551385No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1440.27718426979268923No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1440.27718426979268923No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1440.27718426979268923No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1440.27718426979268923No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1430.27525937903024No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1420.27333448826779083No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1420.27333448826779083No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.2694847067428923No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1390.2675598159804431No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1350.2598602529306462No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1340.25793536216819696No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1340.25793536216819696No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1330.25601047140574773No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1310.2521606898808493No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1290.24831090835595082No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1210.23291178225635698No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1200.23098689149390772No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1160.2232873284441108No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1130.21751265615676307No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1080.20788820234451696No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1070.2059633115820677No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1060.20403842081961848No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1040.20018863929472003No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1010.19441396700737235No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1000.1924890762449231No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1000.1924890762449231No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA990.1905641854824739No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA990.1905641854824739No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA970.1867144039575754No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA970.1867144039575754No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA960.18478951319512618No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA950.18286462243267693No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA940.18093973167022773No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA930.17901484090777847No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA920.17708995014532924No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA870.16746549633308308No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA870.16746549633308308No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA870.16746549633308308No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA850.16361571480818463No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA840.16169082404573543No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA830.15976593328328617No Hit
GGACTACAAGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCCTCA820.15784104252083694No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA820.15784104252083694No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA810.15591615175838772No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA790.15206637023348926No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA790.15206637023348926No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA770.14821658870859078No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCA760.14629169794614155No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA760.14629169794614155No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA740.1424419164212431No Hit
GGACTACAAGGGTTTCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCCTCA740.1424419164212431No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA740.1424419164212431No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA710.13666724413389542No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA710.13666724413389542No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA710.13666724413389542No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA700.13474235337144616No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA680.1308925718465477No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA650.12511789955920002No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA640.12319300879675078No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA640.12319300879675078No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA630.12126811803430154No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA630.12126811803430154No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA620.11934322727185233No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCA620.11934322727185233No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA590.11356855498450462No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA590.11356855498450462No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA580.1116436642220554No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA560.10779388269715694No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA540.10394410117225848No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA540.10394410117225848No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA530.10201921040980924No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA520.10009431964736001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAC255.2023097E-10295.283231
GGACTAA157.1731447E-6295.283231
ACTCGGG4250.0294.999056
AATTAGT206.128721E-8294.99905295
CATTAGC400.0294.99905295
ACGGGTT2900.0294.999058
CTCGGGT4200.0294.999057
TGGTGTA108.4419025E-4294.999058
TACTCGG4250.0294.999055
GTTACCC108.4419025E-4294.99905295
GGTGTAT108.4419025E-4294.999059
AGGGGTA3100.0294.999028
TACAGGG5250.0294.999025
ATTAGCC600.0294.99902295
ACAAGGG9800.0294.999026
CAAGGGT9800.0294.999027
TAGGGTA3300.0294.999028
ACAGGGG5200.0294.999026
ACTACAA9900.0294.999023
GGACTAC48200.0294.67061
Produced by FastQC (version 0.11.5)