FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94064
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCT350.0295.000031
GCGCCTA800.0295.02
CGTTCCT450.0295.01
GACCCCT206.1549144E-8295.01
AGTGCCT206.1549144E-8295.01
TACGCCT303.6379788E-12295.01
ACTCGGA108.4526965E-4295.04
CGAGCCT206.1549144E-8295.01
CCGACCT303.6379788E-12295.01
CGCACCT206.1549144E-8295.01
CGATCCT206.1549144E-8295.01
GCCTACG15900.0290.361634
CCCTACG21700.0290.241944
ACCTACG27400.0290.15514
CTACGGG86400.0290.04926
GCCCTAC5350.0289.485963
GGCCTAC4550.0288.516483
ACGGGAG86000.0288.139538
CGTCCTA2150.0288.139532
CGCCTAC4250.0288.058843
Produced by FastQC (version 0.11.5)