FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78639
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1320.1678556441460344No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1260.1602258421393965No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1240.1576825748038505No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1190.15132440646498557No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1180.15005277279721257No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1170.14878113912943958No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1130.14369460445834764No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1130.14369460445834764No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1120.14242297079057464No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1120.14242297079057464No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1110.14115133712280167No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1110.14115133712280167No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1100.13987970345502868No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1080.1373364361194827No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1060.1347931687839367No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1060.1347931687839367No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1050.13352153511616374No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1050.13352153511616374No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1040.13224990144839074No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1030.13097826778061775No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1030.13097826778061775No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1030.13097826778061775No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1020.12970663411284478No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1020.12970663411284478No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1010.12843500044507178No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1010.12843500044507178No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1010.12843500044507178No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1010.12843500044507178No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1000.1271633667772988No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC1000.1271633667772988No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC990.1258917331095258No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC990.1258917331095258No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC990.1258917331095258No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC980.12462009944175281No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC980.12462009944175281No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC980.12462009944175281No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC970.12334846577397984No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC970.12334846577397984No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC970.12334846577397984No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC970.12334846577397984No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC960.12207683210620686No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC960.12207683210620686No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC950.12080519843843385No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC940.11953356477066086No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC940.11953356477066086No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC930.11826193110288788No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC930.11826193110288788No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC930.11826193110288788No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC920.11699029743511488No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC920.11699029743511488No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC920.11699029743511488No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC920.11699029743511488No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC920.11699029743511488No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC920.11699029743511488No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC910.1157186637673419No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC910.1157186637673419No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC910.1157186637673419No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC900.11444703009956891No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC900.11444703009956891No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC890.11317539643179593No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC890.11317539643179593No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC890.11317539643179593No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC890.11317539643179593No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC890.11317539643179593No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC890.11317539643179593No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC880.11190376276402293No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC880.11190376276402293No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC880.11190376276402293No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC880.11190376276402293No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC870.11063212909624995No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC870.11063212909624995No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC870.11063212909624995No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC870.11063212909624995No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC860.10936049542847696No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC860.10936049542847696No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC860.10936049542847696No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC860.10936049542847696No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC860.10936049542847696No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC860.10936049542847696No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC860.10936049542847696No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC860.10936049542847696No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC850.10808886176070398No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC850.10808886176070398No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC850.10808886176070398No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC850.10808886176070398No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC840.10681722809293098No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC840.10681722809293098No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC840.10681722809293098No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC840.10681722809293098No Hit
AAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.105545594425158No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.105545594425158No Hit
AGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.105545594425158No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.105545594425158No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.105545594425158No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.105545594425158No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.105545594425158No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC820.10427396075738501No Hit
ATGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC820.10427396075738501No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC810.10300232708961203No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC810.10300232708961203No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC810.10300232708961203No Hit
GGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC810.10300232708961203No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC810.10300232708961203No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC810.10300232708961203No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC800.10173069342183903No Hit
CCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC800.10173069342183903No Hit
AACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC800.10173069342183903No Hit
GATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC800.10173069342183903No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC800.10173069342183903No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC800.10173069342183903No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC790.10045905975406605No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC790.10045905975406605No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC790.10045905975406605No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA700.0294.995542
GCCCCTA850.0294.995542
GCCCCCT108.4504404E-4294.995541
ATCCTCG108.4504404E-4294.995543
GTCGCCT108.4504404E-4294.995541
TGCCCCT206.149094E-8294.995541
AGATCCT350.0294.995541
CGAACCT550.0294.995541
CTTCCCT350.0294.995541
CAGCCTA900.0294.995542
CGGCCTA800.0294.995542
TTTAAAC108.4504404E-4294.99554295
GGTCCTA600.0294.995542
TTGTCCT350.0294.995541
CGCGCCT255.275069E-10294.995541
TGAACCT450.0294.995541
GCGCCTA1100.0294.995542
TAGCCTA850.0294.995542
CATTCCT108.4504404E-4294.995541
TAGCCCT108.4504404E-4294.995541
Produced by FastQC (version 0.11.5)