FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83313
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC850.10202489407415409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT303.6379788E-12295.01
TCGCCCT350.0295.01
GCCCCCT157.2163275E-6295.01
GTCGCCT400.0295.01
CGAACCT303.6379788E-12295.01
ACGCCCT450.0295.01
CTATCCT650.0295.01
CTCCCTA1050.0295.02
GGTCCTA500.0295.02
TCCCTAC4450.0295.03
CACGCCT206.150731E-8295.01
CGCGCCT255.275069E-10295.01
GCGCCTA500.0295.02
TCCTCGG108.450965E-4295.04
GACCCTA1000.0295.02
GACCCCT157.2163275E-6295.01
GCACCTA1350.0295.02
GCCGCCT157.2163275E-6295.01
GCACCCT350.0295.01
AACGGGA108.450965E-4295.07
Produced by FastQC (version 0.11.5)