FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTGAAGCAGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGT10.6622516556291391No Hit
GTCGTCACAGGTTGCGCCGCCAAAACGTCGGCTACAGTAACTTTTCCCAG10.6622516556291391No Hit
TTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTT10.6622516556291391No Hit
TCTGCTTCAATATCTGGTTGCACGGCGTCGCGTCGTAACCCAGCTTGGTA10.6622516556291391No Hit
AAATTCAGCGCCTTCCATGATGAGACAGGCCGTTTGAATGTTGACGGGAT10.6622516556291391No Hit
CCGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC10.6622516556291391No Hit
ATACCAAAGACGAGCGCCTTTACGCTTGCCTTTAGTACCTCGCAACGGCT10.6622516556291391No Hit
TGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTG10.6622516556291391No Hit
GTTTCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTT10.6622516556291391No Hit
GCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC10.6622516556291391No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC10.6622516556291391No Hit
AAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGC10.6622516556291391No Hit
ACCCTAAATTTTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCG10.6622516556291391No Hit
CGTCGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGT10.6622516556291391No Hit
TTCGATTTAATTCGTAAACACGCAGTAGTAATTCCTGCTTTATCAAGATA10.6622516556291391No Hit
TTGAACACTCATCCTTAATACCTTTCTTTTTGGGGTAATTATACTCATCG10.6622516556291391No Hit
TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATGATTCAC10.6622516556291391No Hit
TAGGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCATGCTCAGGA10.6622516556291391No Hit
TTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGAC10.6622516556291391No Hit
CGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTTATG10.6622516556291391No Hit
CCATGATGTTATTTCTTCATTTGGAGGTAAAACCTCTTATGACGCTGACA10.6622516556291391No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTC10.6622516556291391No Hit
GGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGT10.6622516556291391No Hit
AGAAAACCTCCCGCGCTTCGCTTGGTCAACCCCTCAGCGGCAAAAATTAA10.6622516556291391No Hit
TTTACCGCTCCTACATGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAA10.6622516556291391No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC10.6622516556291391No Hit
AATGCTTAGGGATTTTATTGGTATCAGGGTTAATCGTGCCAAGAAAAGCG10.6622516556291391No Hit
TTTGTTAACGTATTTAGCCACATAGAAACCACCAGCCATATAACTGGTAG10.6622516556291391No Hit
GAACGCTCGACGCCATTAATAATGTTTTCCGTAAATTCAGCGCCTTCCAT10.6622516556291391No Hit
CCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGCTCG10.6622516556291391No Hit
GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGC10.6622516556291391No Hit
GTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGC10.6622516556291391No Hit
GTCAAAATAATCAGCGTGACATTCAGAAGGGTAATAAGAACGAACCATAA10.6622516556291391No Hit
ATCTAAACCAGTCCTTGACGAACGTGCCAAGCATATTAAGCCACTTCTCC10.6622516556291391No Hit
CGTATCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACTCT10.6622516556291391No Hit
ATTTCTTACCTATTAGTGGTTGAACAGCATCGGACTCAGATAGTAATCCA10.6622516556291391No Hit
ACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGAGACTCAAAAAG10.6622516556291391No Hit
GAATGCAATGCGACAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACA10.6622516556291391No Hit
GACGCCATTAATAATGTTTTCCGTAAATTCAGCGCCTTCCATGATGAGAC10.6622516556291391No Hit
AGACCAGGTATATGCACAAAATGAGATGCTTGCTTATCAACAGAAGGAGT10.6622516556291391No Hit
CACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTA10.6622516556291391No Hit
AATTCCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA10.6622516556291391No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTC10.6622516556291391No Hit
AAGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC10.6622516556291391No Hit
GTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTG10.6622516556291391No Hit
TGTTCCTACGGGAGGCAGCAGGCAGCCTCGAAGGGAGTGAGAGAGGCAAT10.6622516556291391No Hit
AATCCCTACGGGAGGCAATGTGCTCTTCCGATCTGGACTACCCGTGTAGT10.6622516556291391No Hit
AAAATGAGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGCGTTGCG10.6622516556291391No Hit
GGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCATGCTCAGGAACAAA10.6622516556291391No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC10.6622516556291391No Hit
GCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAA10.6622516556291391No Hit
GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGTCCCTAGTTT10.6622516556291391No Hit
TTCTTGGTCAGTATGCAAATTAGCATAAGCAGCTTGCAGACCCATAATGT10.6622516556291391No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC10.6622516556291391No Hit
TCTACCTACGGGAGGCAGCAGTGGGGTATTTTGGACAATGGGGGCAACCC10.6622516556291391No Hit
AATCGTGCCAAGAAAAGCGGCATGGTCAATATAACCAGTAGTGTTAACAG10.6622516556291391No Hit
GCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC10.6622516556291391No Hit
ATCATAAAACGCCTCTAATCGGTCGTCAGCCCACGTGAGAGTGTCAAAAA10.6622516556291391No Hit
ACCTTCTTGAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATTTG10.6622516556291391No Hit
CCGTTCTGGTGATTCGTCTACGAAGTTTAAGATTGCTGAGGGTCAGTGGT10.6622516556291391No Hit
ATTATACTCATCGCGAATATCCTTAAGAGGGCGTTCAGCAGCCAGCTTGC10.6622516556291391No Hit
TGAGCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC10.6622516556291391No Hit
GAGCCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTC10.6622516556291391No Hit
GCCGAATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCA10.6622516556291391No Hit
GTGGACAGATTTGTCATTGTGAGCATTTTCATCCCGAAGTTGCGGCTCAT10.6622516556291391No Hit
GGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCC10.6622516556291391No Hit
TCCCTCATCGTCACGTTTATAGTGAACAGTGGATTAAGTTCATGAAGGAT10.6622516556291391No Hit
GTAGCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC10.6622516556291391No Hit
ATGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGC10.6622516556291391No Hit
CTCCCCTACGGGAGGCAGCAATGAGGAATATTGGTCAATGGGCGTAAGCC10.6622516556291391No Hit
CAAGAAGTCCTTTACCAGCTTTAGCCATAGCACCAGAAACAAAACTAGGG10.6622516556291391No Hit
CACTATGAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGATGAATGCAA10.6622516556291391No Hit
CTGGCCTAACGACGTTTGGTCAGTTCCATCAACATCATAGCCCGATGCCC10.6622516556291391No Hit
GGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGAA10.6622516556291391No Hit
GCAAGCTGGCTGCTGAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT10.6622516556291391No Hit
GTCTTCTTCGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTT10.6622516556291391No Hit
CCAGATATTGAAGCAGACCGCAAAAAGAGAGACGAGATTGAGGCTGGGAA10.6622516556291391No Hit
GTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTT10.6622516556291391No Hit
TATTTAACTGGCGGCGATTGCGTACCCGACGACCAAAATTAGGGTCAACG10.6622516556291391No Hit
AGCTGCGCAAGGATAGGTCGAATTTTCTCATTTTCCGCCAGCAGTCCACT10.6622516556291391No Hit
CCGCAAGCTGGCTGCTGAACGCCCTCTTAAGGATATTCGCGATGAGTATA10.6622516556291391No Hit
ATTACTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTGT10.6622516556291391No Hit
TCATCCTACGGGAGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCT10.6622516556291391No Hit
ATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGACT10.6622516556291391No Hit
GGACTACATGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA10.6622516556291391RNA PCR Primer, Index 29 (96% over 29bp)
TGTAGGCATGGGTGATGCTGGTATTAAATCTGCCATTCAAGGCTCTAATG10.6622516556291391No Hit
GAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTGTCCACGG10.6622516556291391No Hit
CTGGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTATG10.6622516556291391No Hit
ATTACCCTTTCAACTACTCCGGTTATCGCTGGCGACTCCTTCGAGATGGA10.6622516556291391No Hit
CAATCCGCGGCATTTAGTAGCGGTAAAGTTAGACCAAACCATGAAACCAA10.6622516556291391No Hit
CATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTTCGATTTTC10.6622516556291391No Hit
GCCATTAATAATGTTTTCCGTAAATTCAGCGCCTTCCATGATGAGACAGG10.6622516556291391No Hit
GGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCC10.6622516556291391No Hit
ATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCATGATGTTATT10.6622516556291391No Hit
CTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGAA10.6622516556291391No Hit
GAGGCTAACCCTAATGAGCTTAATCAAGATGATGCTCGTTATGGTTTCCG10.6622516556291391No Hit
CCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTG10.6622516556291391No Hit
ATAATGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAA10.6622516556291391No Hit
ACTATCAAAATCTAACGTTGACGATGTAGCTTTAGGTGTCTGTAAAACAG10.6622516556291391No Hit
GTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTGTATGGCAAC10.6622516556291391No Hit
GAGGAGTGGCATTAACACCATCCTTCATGAACTTAATCCACTGTTCACCA10.6622516556291391No Hit
AAGATTGCTGGAGGCCTCCACTATGAAATCGAGTAGAGGCTTTGCTATTC10.6622516556291391No Hit
TAGGGATTTTATTGGTATCAGGGTTAATCGTGCCAAGAAAAGCGGCATGG10.6622516556291391No Hit
TTTTGATTCTCAAATCCGGCGTCAACCATACCAGCAGAGGAAGCATCAGC10.6622516556291391No Hit
TATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTT10.6622516556291391No Hit
ATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCAC10.6622516556291391No Hit
ACAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC10.6622516556291391No Hit
TATCAACAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACAC10.6622516556291391No Hit
CACCATCATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGG10.6622516556291391No Hit
TACTGATAGCAGTCGGCGTGTGAATCATTAGCCTTGCGACCCTCGGCAGC10.6622516556291391No Hit
TCACAGGTTGCGCCGCCAAAAAGTCGGCTACAGTAACTTTTCCCAGCCTC10.6622516556291391No Hit
ATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCG10.6622516556291391No Hit
AGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCCAGAAT10.6622516556291391No Hit
ATGGCAGCAACGGAAACCATAACGAGCATCATCTTGATTAAGCTCATTAG10.6622516556291391No Hit
TCAGTTCCATCAACATCATAGCCAGATGCCCAGAGATTAGAGCGCATGAC10.6622516556291391No Hit
AGAACCTACGGGAGGCAGCAGAGTTCAGACAAGGCAGCCACAGCAGCAAG10.6622516556291391No Hit
AGAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAATTGTTCCAAGTAT10.6622516556291391No Hit
CTAATCGGTCGTCAGCCAACGTGAGAGTGTCAAAAACGATAAACCAACCA10.6622516556291391No Hit
CTTCGCTTGGTCAACCCCTCAGCGGCAAAAATTAAAATTTTTACCGCTTC10.6622516556291391No Hit
GGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGT10.6622516556291391No Hit
CTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG10.6622516556291391No Hit
CTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGACCAGGTATAT10.6622516556291391No Hit
ATGCGGCATACGCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTC10.6622516556291391No Hit
TGCGGACGACCAGGGCGCGCGCCAGAACGTTTTTTACCTTTAGACATTAC10.6622516556291391No Hit
TAAGCATTTGGCGCATAATCTCGGAAACCTGCTGTTGCTTGGAAAGATTG10.6622516556291391No Hit
AGCTCCTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTTAACGT10.6622516556291391No Hit
ACGTGCCAAGCATATTAAGCCACTTCTCCTCATCCAACGCGTCAGTTTTT10.6622516556291391No Hit
TAGAAACCAACAGCCATATAACTGGTAGCTTTAAGCGGCTCACCTTTAGC10.6622516556291391No Hit
TAATCACGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCC10.6622516556291391No Hit
GATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATGATTC10.6622516556291391No Hit
GTTATTTCTTCATTTGGAGGTAAAACCTCTTATGACGCTGACAACCGTCC10.6622516556291391No Hit
TGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTC10.6622516556291391No Hit
AATTTCACGCGGCGGCAAGTTGCCATACAAAACAGGGTCGCCAGCAATAT10.6622516556291391No Hit
GGTGCACTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG10.6622516556291391No Hit
GTTTTATGATAATCCCAATGCTTTACGTGACTATTTTCGTGATATTGGTC10.6622516556291391No Hit
TCTACCTACGGGAGGCAGCAGCGCACCGTGACCCTGGCTTACTACGGTAA10.6622516556291391No Hit
TCATTCTGATTCTGAACAGCTTCTTGGGAAGTAGCGACAGCTTGGTTTTT10.6622516556291391No Hit
CCACCCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA10.6622516556291391No Hit
TAGACCAAACCATGAAACCAACATAAACATTATTGCCCGGCGTACGGGGA10.6622516556291391No Hit
TGGCGGCGATTGCGTACCCGACGACCAAAATTAGGGTCAACGCTACCTGT10.6622516556291391No Hit
CATTATTGCCCGGCGTACGGGGAAGGACGTCAATAGTCACACCGTCCTTG10.6622516556291391No Hit
GCTGAACGCCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAA10.6622516556291391No Hit
GTATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTT10.6622516556291391No Hit
TAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTTGA10.6622516556291391No Hit
TAATGGTGATATGTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGTG10.6622516556291391No Hit
TGGGAAGTAGCGACAGCTTGGTTTTTAGTGAGTTGTTCCATTCTTTAGCT10.6622516556291391No Hit
TTTTAGTAAGCTCTTTTTGATTCTCAAATCCGGCGTCAACCATACCAGCA10.6622516556291391No Hit
AGACCCTACGGGAGGCAGCAGTAAGCAGAAGACGGCATATTAGATACCCT10.6622516556291391No Hit
CGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTG10.6622516556291391No Hit
GAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATAT10.6622516556291391No Hit
GGAACCGAAGAAGACTCAAAGCGAACCAAACAGGCAAAAAATTTAGGGTC10.6622516556291391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)