FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90617
Sequences flagged as poor quality0
Sequence length301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1400.1544963969233146No Hit
TCATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1340.14787512276945827No Hit
TCCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1260.13904675723098314No Hit
CTCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1250.13794321153867375No Hit
TTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1240.13683966584636437No Hit
TTCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1190.13132193738481743No Hit
TCGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1160.12801130030788924No Hit
TTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1150.12690775461557985No Hit
TAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1140.12580420892327046No Hit
TACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1140.12580420892327046No Hit
CACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1130.12470066323096109No Hit
CTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1120.1235971175386517No Hit
ATAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1120.1235971175386517No Hit
ATGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1120.1235971175386517No Hit
CTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1110.1224935718463423No Hit
TACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1100.12139002615403291No Hit
TCCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1090.12028648046172351No Hit
ACATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1090.12028648046172351No Hit
GTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1080.11918293476941412No Hit
TCTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1080.11918293476941412No Hit
TTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1070.11807938907710473No Hit
ATCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1070.11807938907710473No Hit
TTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1070.11807938907710473No Hit
TTGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1070.11807938907710473No Hit
CCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1060.11697584338479536No Hit
CTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1050.11587229769248597No Hit
TAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1050.11587229769248597No Hit
ATATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1050.11587229769248597No Hit
TTACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1040.11476875200017657No Hit
ATCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1040.11476875200017657No Hit
CAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1040.11476875200017657No Hit
AACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1030.11366520630786718No Hit
ACCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1030.11366520630786718No Hit
CTACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1030.11366520630786718No Hit
CCATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1020.11256166061555778No Hit
AAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1020.11256166061555778No Hit
AGATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1010.11145811492324839No Hit
TCTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1010.11145811492324839No Hit
CTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1010.11145811492324839No Hit
TCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1000.110354569230939No Hit
AAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT990.10925102353862963No Hit
TCGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT990.10925102353862963No Hit
CATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT990.10925102353862963No Hit
ACCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT980.10814747784632023No Hit
CTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT970.10704393215401084No Hit
CAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT970.10704393215401084No Hit
AAACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT970.10704393215401084No Hit
TGGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT970.10704393215401084No Hit
GTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT960.10594038646170145No Hit
AATTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT960.10594038646170145No Hit
GGCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT950.10483684076939205No Hit
ATCGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT950.10483684076939205No Hit
AGGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT950.10483684076939205No Hit
TATTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT950.10483684076939205No Hit
AACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT950.10483684076939205No Hit
GTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT950.10483684076939205No Hit
AATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT940.10373329507708266No Hit
CTTGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT940.10373329507708266No Hit
GCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT940.10373329507708266No Hit
CATTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT940.10373329507708266No Hit
TTGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT940.10373329507708266No Hit
ACTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT940.10373329507708266No Hit
ATTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT930.10262974938477328No Hit
CTGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT930.10262974938477328No Hit
TTCCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT930.10262974938477328No Hit
GGTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT920.10152620369246387No Hit
ACGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT920.10152620369246387No Hit
GTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT920.10152620369246387No Hit
GTCCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT910.1004226580001545No Hit
CCCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT910.1004226580001545No Hit
TAGCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT910.1004226580001545No Hit
GACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT910.1004226580001545No Hit
GGGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT910.1004226580001545No Hit
CACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT910.1004226580001545No Hit
TTGGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT910.1004226580001545No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCT350.0295.000031
GGATCCT157.218414E-6295.01
TACGAGG157.218414E-6295.07
CGGGCCT206.153641E-8295.01
CGAGCCT157.218414E-6295.01
CCGACCT400.0295.01
GGCGCCT206.153641E-8295.01
TAGTCCT450.0295.01
GCCTACG16900.0291.508884
CCTACGG84700.0290.64645
CTACGGG83050.0290.55996
ACGGGAG81800.0290.311748
TACGGGA81950.0290.140357
ACCTACG24700.0289.625524
TCCTACG23750.0289.410524
CCCTACG19950.0288.345864
CGGGAGG80300.0287.652569
CGTCCTA1600.0285.781252
GACCTAC5750.0284.739143
ACCCTAC5250.0283.76193
Produced by FastQC (version 0.11.5)