FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84880
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15961.880301602262017No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15061.7742695570216775No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10551.2429311969839774No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10491.2358623939679547No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10261.2087653157398681No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10231.2052309142318567No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10221.204052780395853No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10081.1875589066918002No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9401.1074458058435437No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8811.0379359095193215No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7690.9059849198868991No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7350.865928369462771No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7180.8459000942507069No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7150.8423656927426957No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7010.8258718190386427No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6860.8081998114985863No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6840.8058435438265787No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6740.794062205466541No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6530.7693213949104618No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6410.7551837888784165No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6350.748114985862394No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6300.7422243166823751No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5820.6856738925541942No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5550.6538642789820924No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5540.6526861451460886No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5440.6409048067860509No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5290.6232327992459943No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5030.5926013195098963No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5020.5914231856738925No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4940.5819981149858624No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4930.5808199811498587No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4920.5796418473138548No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4790.5643261074458059No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4660.5490103675777568No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4600.5419415645617343No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4350.51248821866164No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4350.51248821866164No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4280.5042412818096136No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4200.4948162111215834No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4190.4936380772855796No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4170.4912818096135721No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4060.47832233741753066No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4050.4771442035815269No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3970.46771913289349665No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3960.4665409990574929No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3930.4630065975494816No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3860.4547596606974552No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3810.4488689915174364No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3770.4441564561734213No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3690.4347313854853912No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3680.43355325164938735No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3590.42295004712535345No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3580.42177191328934965No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3510.4135249764373233No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3410.4017436380772856No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3360.3958529688972668No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3360.3958529688972668No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3250.3828934967012253No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3210.3781809613572102No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3130.36875589066918No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3090.36404335532516496No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3080.36286522148916117No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3030.3569745523091423No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3000.353440150801131No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3000.353440150801131No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2950.34754948162111216No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2910.3428369462770971No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2910.3428369462770971No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2910.3428369462770971No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2840.33459000942507067No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2760.32516493873704055No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2750.32398680490103676No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2710.31927426955702165No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2690.3169180018850141No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2660.3133836003770028No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2610.307492931196984No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2590.3051366635249764No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2580.3039585296889727No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2570.3027803958529689No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2510.29571159283694626No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2470.2909990574929312No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2470.2909990574929312No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2460.2898209236569274No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2420.28510838831291235No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2370.27921771913289345No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2300.27097078228086713No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2280.26861451460885954No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2220.26154571159283696No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2170.25565504241281806No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2070.24387370405278042No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2060.24269557021677662No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2060.24269557021677662No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2030.23916116870876533No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2010.23680490103675775No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1980.23327049952874646No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1970.2320923656927427No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1870.22031102733270502No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1860.21913289349670123No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1850.21795475966069747No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1850.21795475966069747No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1770.2085296889726673No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1740.20499528746465598No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1530.1802544769085768No Hit
GGACTACACGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1500.1767200754005655No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1480.17436380772855797No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1480.17436380772855797No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1440.16965127238454286No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1320.15551366635249764No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1300.1531573986804901No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1280.15080113100848258No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1270.1496229971724788No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1240.14608859566446747No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1230.1449104618284637No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1230.1449104618284637No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1220.14373232799245994No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1220.14373232799245994No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1200.1413760603204524No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1170.1378416588124411No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1160.13666352497643733No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1160.13666352497643733No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1110.13077285579641848No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1100.12959472196041472No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.12370405278039585No Hit
GGACTACACGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1050.12370405278039585No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1050.12370405278039585No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1040.12252591894439209No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1030.12134778510838831No Hit
GGACTACACGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1020.12016965127238455No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1020.12016965127238455No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1010.11899151743638076No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1010.11899151743638076No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC970.11427898209236569No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA970.11427898209236569No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.11310084825636192No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC950.11192271442035816No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA950.11192271442035816No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC930.10956644674835062No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC890.10485391140433553No Hit
GGACTACCCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC890.10485391140433553No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA890.10485391140433553No Hit
GGACTACTCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC890.10485391140433553No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC880.10367577756833177No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA870.10249764373232799No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA850.10014137606032046No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA850.10014137606032046No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACTA108.451241E-4295.02
TTGGGTA108.451241E-4295.08
TGACTAC157.2168023E-6294.999971
GGACTAC81850.0294.81981
CACGGGT15000.0294.016667
TACACGG14950.0294.013375
GACTACC24050.0293.773382
CAAGGGT9350.0293.42257
ACGGGTA7550.0293.04648
ACACGGG15000.0293.033336
CTACCGG7200.0292.95144
TCGGGTT5300.0292.216988
CTACTCG10200.0292.107854
TACTCGG10200.0292.107855
ACTCGGG10150.0292.09366
ACGGGTT7550.0291.092748
GACTACA35500.0290.84512
CTACCCG10400.0290.74524
CGGGGTA3450.0290.724648
ACCCGGG10350.0290.72466
Produced by FastQC (version 0.11.5)