FastQCFastQC Report
Wed 8 May 2024
EGAF00005492363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50620
Total Bases15.2 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.12445673646779928No Hit
TATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT610.12050572896088502No Hit
TTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT570.1126037139470565No Hit
TCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT570.1126037139470565No Hit
TTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT560.11062821019359936No Hit
TATTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT540.10667720268668511No Hit
TCATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT530.10470169893322798No Hit
AAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT530.10470169893322798No Hit
ATCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT530.10470169893322798No Hit
TAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT530.10470169893322798No Hit
TTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT520.10272619517977084No Hit
CCATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT520.10272619517977084No Hit
TACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT520.10272619517977084No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)