FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50620
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.12445673646779928No Hit
TATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT610.12050572896088502No Hit
TTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT570.1126037139470565No Hit
TCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT570.1126037139470565No Hit
TTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT560.11062821019359936No Hit
TATTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT540.10667720268668511No Hit
TCATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT530.10470169893322798No Hit
AAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT530.10470169893322798No Hit
ATCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT530.10470169893322798No Hit
TAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT530.10470169893322798No Hit
TTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT520.10272619517977084No Hit
CCATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT520.10272619517977084No Hit
TACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT520.10272619517977084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA350.0295.02
CATATCG108.4411725E-4295.0295
ATCTACG108.4411725E-4295.03
TCCCTAG108.4411725E-4295.03
GGATCCT400.0295.01
TACGGCA108.4411725E-4295.07
TTCCCTA900.0295.02
TCGGAAA108.4411725E-4295.0295
AGCGCCT108.4411725E-4295.01
TCGTCCT255.2386895E-10295.01
CTCGCCT108.4411725E-4295.01
CCGACCT108.4411725E-4295.01
GCGTCCT108.4411725E-4295.01
CGCCCTA450.0295.02
TAGACCT303.6379788E-12294.999971
TGAACCT157.199613E-6294.999971
GGCGCCT157.199613E-6294.999971
CCCTACG11650.0293.733924
ACCTACG13200.0291.647744
CCTACGG48400.0290.42875
Produced by FastQC (version 0.11.5)