FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54853
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC590.10756020636975187No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC580.10573715202450186No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC560.10209104333400178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCTA950.0295.000032
TCGCCTA750.0295.02
TCGCCCT255.2386895E-10295.01
AGATCCT303.6379788E-12295.01
CGAACCT157.2029106E-6295.01
TCCGCCT157.2029106E-6295.01
ATAGCCT255.2386895E-10295.01
GCGCCTA700.0295.02
AGAACCT600.0295.01
AAGCCTA900.0295.02
TACGCCT303.6379788E-12295.01
TGCGCCT303.6379788E-12295.01
CCCCCTA1000.0295.02
GAGCCTA303.6379788E-12295.02
GGGCCTA850.0295.02
CTCGCCT303.6379788E-12295.01
CGTACCT157.2029106E-6295.01
TCTCCCT350.0295.01
ACGTCCT255.2386895E-10295.01
CCGACCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)