FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76350
Sequences flagged as poor quality0
Sequence length301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA550.0295.000032
TCGCCTA850.0295.02
ACGCCTA750.0295.02
CACGCCT108.449585E-4295.01
GCGCCCT157.2139737E-6295.01
CACCTAG108.449585E-4295.03
TTCCCTA1000.0295.02
TACGCCT255.2568794E-10295.01
CGCTCCT255.2568794E-10295.01
GTTCCCT157.2139737E-6295.01
GAGCCTA950.0295.02
CTCGCCT108.449585E-4295.01
CGAGCCT450.0295.01
TTGCCTA800.0295.02
GACGCCT206.1474566E-8295.01
GGCGCCT157.2139737E-6295.01
CGTCCTA800.0295.02
CGATCCT157.2139737E-6295.01
ATCGCCT157.2139737E-6295.01
CGCCCTA1050.0295.02
Produced by FastQC (version 0.11.5)