FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103880
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT500.0294.996641
GCCCCCT157.2217827E-6294.996641
AATCCTA1850.0294.996642
AAATCCT305.456968E-12294.996641
AGATCCT550.0294.996641
CGAACCT157.2217827E-6294.996641
GCGACCT550.0294.996641
TCCGCCT350.0294.996641
GAGTCCT350.0294.996641
ACACCCT500.0294.996641
GATCCTA2000.0294.996642
TTGTCCT550.0294.996641
GATCCCT305.456968E-12294.996641
CACGCCT305.456968E-12294.996641
CCTACGA157.2217827E-6294.996645
GCGCCCT305.456968E-12294.996641
TAGCCCT305.456968E-12294.996641
GACCCCT350.0294.996641
AGGCCTA950.0294.996642
TGACCTA1500.0294.996642
Produced by FastQC (version 0.11.5)