FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51827
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC550.10612229146969726No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC530.10226329905261736No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCTA850.0295.000032
GACCTAC3250.0295.000033
GCCCCTA650.0295.02
GTACCTA650.0295.02
AGATCCT255.2386895E-10295.01
ACGCCTA650.0295.02
CTTCCCT255.2386895E-10295.01
AGCCCTA350.0295.02
TAGACCT255.2386895E-10295.01
GGTCCTA750.0295.02
CAACCTA550.0295.02
GGTCCCT255.2386895E-10295.01
AGAACCT255.2386895E-10295.01
ACCTCCT350.0295.01
CGCCTAC2150.0295.03
GTTACCT255.2386895E-10295.01
CGGGGGC550.0295.09
ATATCCT255.2386895E-10295.01
TACGCCT255.2386895E-10295.01
CTACGGA1000.0295.06
Produced by FastQC (version 0.11.5)