FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86330
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA550.0295.000032
TGGCCTA1100.0295.000032
CGAACCT350.0295.01
ACGCCTA1050.0295.02
GCGACCT108.451491E-4295.01
CGTGCCT206.152004E-8295.01
GAGTACT108.451491E-4295.0295
CTCCCTA850.0295.02
TTGTCCT650.0295.01
CGCGCCT206.152004E-8295.01
CCTACGA108.451491E-4295.05
TCCTCGG157.217228E-6295.04
GCGCCCT303.6379788E-12295.01
CGTTCCT450.0295.01
ACCCTAC4350.0295.03
GACCCCT500.0295.01
ACCTCGG255.275069E-10295.04
CGGGGCA157.217228E-6295.09
GGGACCT255.275069E-10295.01
GTGTCGG206.152004E-8295.0295
Produced by FastQC (version 0.11.5)