FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73599
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA19632.6671558037473333No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA18872.5638935311621083No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA16822.2853571380045925No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA16772.2785635674397753No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15972.1698664384026953No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15472.101930732754521No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15412.09377844807674No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14001.902199758148888No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13821.877742904115545No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13211.7948613432247722No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11741.595130368619139No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11641.5815432274895038No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11531.5665973722469055No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11121.5108900936154024No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA11091.506813951276512No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10701.4538241008709358No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10671.4497479585320452No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA10551.4334433891764835No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA10281.396758108126469No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9951.351920542398674No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9861.3396921153820025No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA9781.3288224024782946No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9321.266321553281974No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA9271.2595279827171564No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA9081.2337124145708502No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA8601.1684941371486024No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA8521.1576244242448945No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8451.14811342545415No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8331.1318088560985884No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8251.1209391431948803No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8221.1168630008559899No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7731.0502860093207789No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA7320.9945787306892757No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA7290.9905025883503851No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7230.9823503036726043No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA7070.9606108778651885No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6870.9334365956059185No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6730.9144145980244297No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6610.8981100286688679No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6580.8940338863299773No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6520.8858816016521963No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6250.8491963206021821No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6150.8356091794725472No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6120.8315330371336567No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6060.8233807524558757No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5870.7975651843095695No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5780.785336757292898No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5570.7568037609206647No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5530.7513689044688107No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5520.7500101903558473No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5440.7391404774521393No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5070.6888680552724901No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4980.6766396282558187No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4980.6766396282558187No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4880.6630524871261837No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4540.6168562072854251No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4290.5828883544613378No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4120.5597902145409585No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4080.5543553580891045No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3800.5163113629261267No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3790.5149526488131632No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3390.4606040842946236No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3350.45516922784276964No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3060.41576651856682834No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2950.40082066332422994No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2910.39538580687237596No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2860.3885922363075585No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2770.3763638092908871No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2720.36957023872606964No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2700.3668528105001427No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2560.3478308129186538No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2300.312504245981603No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2200.2989171048519681No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2120.2880473919482602No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2110.28668867783529667No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2030.27581896493158875No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2030.27581896493158875No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2000.2717428225926983No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2000.2717428225926983No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1920.2608731096889903No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1860.2527208250112094No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1830.24864468267231893No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1790.24320982622046497No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1740.2364162556556475No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1680.22826397097786655No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1660.22554654275193955No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1640.2228291145260126No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1560.21195940162230467No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1440.19565483226674277No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1420.19293740404081577No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1390.1888612617019253No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1390.1888612617019253No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1390.1888612617019253No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1360.18478511936303482No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1360.18478511936303482No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1300.17663283468525387No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1280.1739154064593269No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1190.16168697944265548No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1160.157610837103765No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.154893408877838No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1120.15217598065191104No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1070.14538241008709357No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1050.1426649818611666No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA990.13451269718338565No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA950.12907784073153167No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA930.1263604125056047No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA920.1250016983926412No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA890.12092555605375073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACTG6150.0295.000033
CGTTCGC108.448955E-4295.0295
CGTTAGA350.0295.0295
CGGGGTA3800.0295.08
AATTATA108.448955E-4295.0295
AATTAGA4000.0295.0295
GCTACTC108.448955E-4295.02
GCTACTA108.448955E-4295.02
TGTTAGC350.0295.0295
GCTACCG108.448955E-4295.02
CATTAGA206.1458195E-8295.0295
AGGCTAT108.448955E-4295.09
AGACTAC108.448955E-4295.01
AGTTCGA206.1458195E-8295.0295
AGTTTGC108.448955E-4295.0295
TCGGGTT3250.0295.08
ATTTAGA303.6379788E-12295.0295
AGTTAAC255.2568794E-10295.0295
AGTTAGA6700.0294.99997295
GGACTAC71600.0294.7941
Produced by FastQC (version 0.11.5)