FastQCFastQC Report
Thu 12 May 2022
EGAF00005492719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67884938
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8334561.2277480462602766TruSeq Adapter, Index 12 (97% over 40bp)
GGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAAC1168220.17208824732225578No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATG878540.1294160421859706No Hit
GGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA810340.11936963100710204No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGCA772160.11374540844391727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)