FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005492764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56425729
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTACGTCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA5691801.008724229331623Illumina Single End PCR Primer 1 (97% over 34bp)
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCC3345700.5929387283591853No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC2485770.4405383933985151No Hit
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGG2018540.35773396919692435No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC1773790.31435836655296023No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACG1725610.3058197085942833No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCG1622140.2874823292048207No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGG1616090.2864101232967677No Hit
CGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGA1382020.24492727422272204No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGG1296410.22975511756347888No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGGCGG1175790.20837834456689078No Hit
GGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCG1111980.19706967365897923No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGA1100390.19501564614256026No Hit
GGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGA981790.17399686586237992No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG923230.16361862156889456No Hit
CGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCG921660.1633403797051519No Hit
GGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGG898660.15926422501337997No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGG796130.14109343629392898No Hit
GGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCGGCTCC795750.14102609112945622No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGC761330.13492603702116104No Hit
GAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGG705420.12501743663781462No Hit
CGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC664030.11768213043379554No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGG656860.1164114335146649No Hit
GGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCC640740.11355458074808392No Hit
GGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCGGCTC586950.10402169549284866No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGC583200.1033571050539728No Hit
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCC577950.10242667843954661No Hit
AGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGG567450.10056582521069422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)