FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887634
Sequences flagged as poor quality0
Sequence length248
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGCAAAGGTCGTGATCTAAACGTTGTAGC22410.25246892300204815No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTAGG12390.13958455850046303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA868900.0241.15587
TCGTGAC871950.0240.930332
GTGACTG872550.0240.69834
TGACTGG875000.0239.943835
TGGGAAA875550.0239.420919
GACTGGG882650.0237.52186
CGTGACT895800.0234.531333
CTGGGAA907300.0230.909278
CGAGACT2100.0190.28613
ACGTGAC2050.0189.094972
TCGAGAC2150.0180.299872
CTGCGAA1550.0148.317268
GTCGTGA1693050.0124.024751
GACGTGA2900.0121.193651
CGGGGAA2350.0118.421299
GACTGGA4450.0116.9303366
GACTGGC1150.0115.7480246
TGCGAAA2050.0112.142339
CGTGGCT3650.0109.4796753
ACTGGAA4800.0108.391957
Produced by FastQC (version 0.11.5)