FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053730
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGATG15080.14311066402209294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA1032600.0241.199557
TCGTGAC1036150.0241.196472
GTGACTG1036600.0240.81574
TGACTGG1037550.0240.57315
TGGGAAA1039200.0239.633019
GACTGGG1046050.0238.329036
CGTGACT1062750.0234.820893
CTGGGAA1082500.0230.070058
CGAGACT2250.0172.176183
CTGCGAA900.0161.323248
TGCGAAA1150.0136.774069
TCGAGAC3150.0130.756332
ACGTGAC2000.0127.1989752
GACTGGA4200.0126.7720266
GTCGTGA2013600.0124.083081
GACGGGA1900.0121.009656
ACCGGGA2300.0115.737387
CGGGGAA2650.0109.578069
GCGTGGC1550.0101.6028752
GTCGAGA3700.0101.52621
Produced by FastQC (version 0.11.5)