FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838186
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCAGAT8580.10236391445335523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT824300.0241.1153
TGACTGG824300.0240.999335
GTGACTG824450.0240.99834
TCGTGAC826150.0240.702942
ACTGGGA826250.0239.933587
GACTGGG827550.0239.658666
CTGGGAA843100.0235.095288
TGGGAAA862450.0229.876749
CGAGACT1850.0137.415853
GTGACTA1150.0136.83054
ACTAGGA1000.0133.0917
GTCGTGA1607600.0123.681941
CGTGGCT2850.0123.1806263
ACGTGAC1950.0111.83822
CGGGGAA1850.0111.181689
TCGTGGC3100.0105.5247652
TGACCGG1700.099.6581655
GAGATCG1100.098.993317
GACTAGG1500.088.7326356
GTCGTCA1250.087.265091
Produced by FastQC (version 0.11.5)