FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095228
Sequences flagged as poor quality0
Sequence length248
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT2001900.0241.848533
TGACTGG2005600.0241.524665
GTGACTG2006350.0241.396294
TCGTGAC2011000.0241.318122
GACTGGG2014800.0240.256276
ACTGGGA2010000.0239.379037
TGGGAAA1977150.0238.791479
CTGGGAA2051750.0235.665638
CGTGGCT34450.0231.400563
TCGTGGC31450.0206.136222
GCGTGGC9450.0205.167862
CGTTACT1050.0196.149573
GCGGGGC9200.0175.180192
GTCGTGG41100.0161.934191
GTGACTT10750.0161.078454
TGACTTG12000.0146.293155
GGCGTGG13850.0146.175551
CGTTACA950.0140.280333
TTGGAAA13550.0140.227029
TCGTTAC2250.0140.027072
Produced by FastQC (version 0.11.5)