FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950799
Sequences flagged as poor quality0
Sequence length248
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGACC23790.12195003175621887No Hit
GTCGTGACTGGGAAAACCCTGGCCAGGAGTCGTGATCTTCTTGACTGACC21920.11236421589307766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA1915800.0241.04547
TCGTGAC1929950.0240.087232
CGTGACT1941100.0238.381293
TGACTGG1942150.0238.185825
GTGACTG1944500.0237.890734
TGGGAAA1943750.0237.691339
GACTGGG1947700.0237.271016
CTGGGAA1971750.0234.242348
ACCGGGA4700.0131.297367
TCGAGAC4800.0123.682042
GTCGTGA3749400.0123.564111
CTCGGAA1800.0120.9995358
CGTGACG3900.0117.954433
CGAGACT5350.0117.664333
GCGTGGC3200.0117.371752
ACTCGGA1900.0114.631147
CTGCGAA1750.0110.628158
GTGACGG4150.0107.909524
GTCGTAA1250.096.976111
CGGGGAA5850.095.1449369
Produced by FastQC (version 0.11.5)