FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2588340
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGTGGCCGTCGTGATACGCGCTGCTAGCT68280.26379841906395607No Hit
GTCGTGACTGGGAAAACCCTGGAACGGCGTCGTGATCAAGGTGAGTAGCT54690.21129372493567306No Hit
GTCGTGACTGGGAAAACCCTGGCCCAACGTCGTGATAGCAAATGCTAGCT54630.21106191613157466No Hit
GTCGTGACTGGGAAAACCCTGGGGGCACGTCGTGATCACTTCCGCTAGCT53680.20739161006668366No Hit
GTCGTGACTGGGAAAACCCTGGCTGGTTGTCGTGATCTGACGTGCTAGCT50980.1969602138822566No Hit
GTCGTGACTGGGAAAACCCTGGTTTATAGTCGTGATGCTACCAAATAGCT49920.19286492500985186No Hit
GTCGTGACTGGGAAAACCCTGGAGTACTGTCGTGATCTGCTAAGCTAGCT48060.1856788520828021No Hit
GTCGTGACTGGGAAAACCCTGGGGATTTGTCGTGATAAGCAGTCATAGCT47860.18490615606914085No Hit
GTCGTGACTGGGAAAACCCTGGTGCCCAGTCGTGATGCTGAGTCGTAGCT47700.18428799925821182No Hit
GTCGTGACTGGGAAAACCCTGGACATCGGTCGTGATATAGTTAAGTAGCT47660.1841334600554796No Hit
GTCGTGACTGGGAAAACCCTGGAACACCGTCGTGATGTCCAGGGATAGCT45590.17613605631408547No Hit
GTCGTGACTGGGAAAACCCTGGGGTATGGTCGTGATGAATGATAGTAGCT43630.16856363538020508No Hit
GTCGTGACTGGGAAAACCCTGGCGTGGAGTCGTGATTCAGCCCCATAGCT43550.16825455697474057No Hit
GTCGTGACTGGGAAAACCCTGGGCCCAAGTCGTGATAGTCCACCGTAGCT43500.16806138297132525No Hit
GTCGTGACTGGGAAAACCCTGGAGCCATGTCGTGATTTTGGTACCTAGCT42180.1629615892811609No Hit
GTCGTGACTGGGAAAACCCTGGCATCCAGTCGTGATCGAGGCTAGTAGCT41950.16207298886545043No Hit
GTCGTGACTGGGAAAACCCTGGGTGATCGTCGTGATATTGAGTAGTAGCT41360.1597935356251497No Hit
GTCGTGACTGGGAAAACCCTGGACTATCGTCGTGATCCGCTGTACTAGCT39260.15168022748170643No Hit
GTCGTGACTGGGAAAACCCTGGCGAACAGTCGTGATGCGAAGCCCTAGCT38560.14897579143389197No Hit
GTCGTGACTGGGAAAACCCTGGCGGGGGGTCGTGATAGCAAAACCTAGCT37510.14491913736217035No Hit
GTCGTGACTGGGAAAACCCTGGGGAAGTGTCGTGATTGCTAGATCTAGCT37120.14341238013553087No Hit
GTCGTGACTGGGAAAACCCTGGCGGGTAGTCGTGATGAATGCAAATAGCT36970.14283285812528493No Hit
GTCGTGACTGGGAAAACCCTGGTATCCCGTCGTGATACAGGATTGTAGCT36840.1423306057164051No Hit
GTCGTGACTGGGAAAACCCTGGTGGAGCGTCGTGATCTACATGAGTAGCT36320.14032159608088582No Hit
GTCGTGACTGGGAAAACCCTGGGTAAGTGTCGTGATGCCGGACTTTAGCT36100.13947163046585842No Hit
GTCGTGACTGGGAAAACCCTGGGACTCTGTCGTGATACAGCAGGCTAGCT35960.13893074325629556No Hit
GTCGTGACTGGGAAAACCCTGGTGCATCGTCGGATCCTCAGTGCGTAGCT35930.13881483885424634No Hit
GTCGTGACTGGGAAAACCCTGGCATATCGTCGTGATATAAATGGCTAGCT35860.1385443952494649No Hit
GTCGTGACTGGGAAAACCCTGGTTACGTGTCGTGATGGGCGTGCGTAGCT35760.13815804724263428No Hit
GTCGTGACTGGGAAAACCCTGGACGGTGGTCGTGATTTCACGACTTAGCT35640.1376944296344375No Hit
GTCGTGACTGGGAAAACCCTGGCTATCAGTCGTGATGCAGTCGGGTAGCT35210.1360331332050658No Hit
GTCGTGACTGGGAAAACCCTGGAGAGATGTCGTGATCAAAACTCATAGCT35090.13556951559686903No Hit
GTCGTGACTGGGAAAACCCTGGGTAAAGGTCGTGATGGCCGATCTTAGCT34960.13506726318798923No Hit
GTCGTGACTGGGAAAACCCTGGGACTATGTCGTGATCTCATCACGTAGCT34920.13491272398525694No Hit
GTCGTGACTGGGAAAACCCTGGCTGACAGTCGTGATGCAAAGAAGTAGCT34680.13398548876886343No Hit
GTCGTGACTGGGAAAACCCTGGCAGCACGTCGTGATCGATTCAGCTAGCT34560.1335218711606667No Hit
GTCGTGACTGGGAAAACCCTGGCCTTTGGTCGTGATAGTGCGGGGTAGCT34500.1332900623565683No Hit
GTCGTGACTGGGAAAACCCTGGCGGGTAGTCGTGTACTACGAGCCTAGCT34300.13251736634290703No Hit
GTCGTGACTGGGAAAACCCTGGTCACTAGTCGTGATGAACATCTCTAGCT34300.13251736634290703No Hit
GTCGTGACTGGGAAAACCCTGGTCCGCCGTCGTGATATAACCGAGTAGCT34240.13228555753880866No Hit
GTCGTGACTGGGAAAACCCTGGGAGACCGTCGTGATGATGCGTGTTAGCT34120.1318219399306119No Hit
GTCGTGACTGGGAAAACCCTGGGACGAAGTCGTGATCATAACCAATAGCT33860.13081743511285227No Hit
GTCGTGACTGGGAAAACCCTGGGCCCGGGTCGTGATCATACGCACTAGCT33680.13012200870055712No Hit
GTCGTGACTGGGAAAACCCTGGGTTCCCGTCGTGATTAGAAAAGCTAGCT33630.1299288346971418No Hit
GTCGTGACTGGGAAAACCCTGGTATTTCGTCGTGATACACATAGATAGCT33520.1295038518896281No Hit
GTCGTGACTGGGAAAACCCTGGGAGACGGTCGTGATCCTAACATCTAGCT33480.12934931268689584No Hit
GTCGTGACTGGGAAAACCCTGGACAGATGTCGTGATGGCGTCTCGTAGCT33130.12799709466298864No Hit
GTCGTGACTGGGAAAACCCTGGGGTCGCGTCGTGATTAACGGGGGTAGCT33010.12753347705479187No Hit
GTCGTGACTGGGAAAACCCTGGTCCTGCGTCGTGATCTATATTCCTAGCT32950.1273016682506935No Hit
GTCGTGACTGGGAAAACCCTGGTGCGAGGTCGTGATACCTGGTCCTAGCT32940.12726303345001044No Hit
GTCGTGACTGGGAAAACCCTGGCTTTGAGTCGTGATGAACGGTATTAGCT32590.12591081542610322No Hit
GTCGTGACTGGGAAAACCCTGGCGGAGGGTCGGGATCCTACACCATAGCT32550.12575627622337096No Hit
GTCGTGACTGGGAAAACCCTGGAGAAACGTCGTGATACCAATCCCTAGCT32550.12575627622337096No Hit
GTCGTGACTGGGAAAACCCTGGTTCCGTGTCGTGATCTGAACTCGTAGCT32470.12544719781790645No Hit
GTCGTGACTGGGAAAACCCTGGGCTTAAGTCGTGATAAGTAGTTCTAGCT32460.1254085630172234No Hit
GTCGTGACTGGGAAAACCCTGGCGTCCCGTCGTGATCAAAAGTCATAGCT32420.12525402381449113No Hit
GTCGTGACTGGGAAAACCCTGGGCAGAGGTCGTGATAGCTGGAACTAGCT32240.124558597402196No Hit
GTCGTGACTGGGAAAACCCTGGAATGTCGTCGTGATGAATACTGCTAGCT32140.12417224939536536No Hit
GTCGTGACTGGGAAAACCCTGGCCGTAAGTCGTGATTGAGCCCGCTAGCT32050.1238245361892178No Hit
GTCGTGACTGGGAAAACCCTGGCACGGGGTCGTGATCCAAGAGAGTAGCT32040.12378590138853474No Hit
GTCGTGACTGGGAAAACCCTGGGGAGCTGTCGTGATGGCAAGAGCTAGCT32010.12366999698648555No Hit
GTCGTGACTGGGAAAACCCTGGCCGGCAGTCGTGATATACTACGGTAGCT31850.12305184017555654No Hit
GTCGTGACTGGGAAAACCCTGGCGGGACGTCGTGATGGTGGCCCGTAGCT31850.12305184017555654No Hit
GTCGTGACTGGGAAAACCCTGGCGGCAAGTCGTGATTCCTTCGCGTAGCT31570.12197006575643077No Hit
GTCGTGACTGGGAAAACCCTGGACATTAGTCGTGATCACAATGAGTAGCT31470.12158371774960014No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGAGTCGTGATGTCCTGAGCTAGCT31460.12154508294891705No Hit
GTCGTGACTGGGAAAACCCTGGAATGACGTCGTGATATCAGTTCCTAGCT31450.121506448148234No Hit
GTCGTGACTGGGAAAACCCTGGGAGCCTGTCGTGATCTGGTACCTTAGCT31380.12123600454345256No Hit
GTCGTGACTGGGAAAACCCTGGTACTGAGTCGTGATATACTATTGTAGCT31340.12108146534072031No Hit
GTCGTGACTGGGAAAACCCTGGGTGCATGTCGTGATCTCGATTCCTAGCT31320.12100419573935418No Hit
GTCGTGACTGGGAAAACCCTGGGTCTCAGTCGTGATTGGTGCAGGTAGCT31310.12096556093867113No Hit
GTCGTGACTGGGAAAACCCTGGTAAGTAGTCGTGATATGCTGCCCTAGCT31260.1207723869352558No Hit
GTCGTGACTGGGAAAACCCTGGGTGGCTGTCGTGATTTGCATAGATAGCT31240.12069511733388968No Hit
GTCGTGACTGGGAAAACCCTGGGAGTATGTCGTGATACGTTGTGCTAGCT31220.12061784773252356No Hit
GTCGTGACTGGGAAAACCCTGGTGGCGCGTCGTGATTTGAACCCCTAGCT31080.12007696052296066No Hit
GTCGTGACTGGGAAAACCCTGGATTCACGTCGTGATGGTTAGTTCTAGCT30950.11957470811408083No Hit
GTCGTGACTGGGAAAACCCTGGACGGGCGTCGTGATGTGTATCGCTAGCT30860.11922699490793326No Hit
GTCGTGACTGGGAAAACCCTGGCGACTTGTCGTGATATGGATTTCTAGCT30830.11911109050588409No Hit
GTCGTGACTGGGAAAACCCTGGATGGTTGTCGTGATATTCGCTGCTAGCT30690.11857020329632119No Hit
GTCGTGACTGGGAAAACCCTGGAACTGAGTCGTGATAGTGGTAGTTAGCT30430.11756569847856155No Hit
GTCGTGACTGGGAAAACCCTGGCGCGTCGTCGTGATAGTTGCCCATAGCT30360.11729525487378009No Hit
GTCGTGACTGGGAAAACCCTGGTCGGCAGTCGTGATTAAAGAAACTAGCT30340.11721798527241398No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGTGTCGTGATAACGTCCGCTAGCT30190.11663846326216802No Hit
GTCGTGACTGGGAAAACCCTGGAGTTGCGTCGTGATGTTGTCGCTTAGCT30090.1162521152553374No Hit
GTCGTGACTGGGAAAACCCTGGCCAATGGTCGTGATGAGCCCCCATAGCT30060.11613621085328821No Hit
GTCGTGACTGGGAAAACCCTGGGAGGTCGTCGTGATACCTGGGGATAGCT30050.11609757605260515No Hit
GTCGTGACTGGGAAAACCCTGGTAACGTGTCGTGATGACCTGTCTTAGCT29930.11563395844440838No Hit
GTCGTGACTGGGAAAACCCTGGCGCCTTGTCGTGATTCCTCGTGATAGCT29910.11555668884304225No Hit
GTCGTGACTGGGAAAACCCTGGCGCGAGGGTCGTGATCCGTGTTCTAGCT29910.11555668884304225No Hit
GTCGTGACTGGGAAAACCCTGGGGATCCGTCGTGATGAGGGACCCTAGCT29900.1155180540423592No Hit
GTCGTGACTGGGAAAACCCTGGAAGATTGTCGTGATCCGAGTGACTAGCT29680.1146680884273318No Hit
GTCGTGACTGGGAAAACCCTGGGCGGCGGTCGTGATTGCAATTACTAGCT29550.11416583601845198No Hit
GTCGTGACTGGGAAAACCCTGGTCGCTCGTCGTGATGACCAAGAGTAGCT29510.11401129681571973No Hit
GTCGTGACTGGGAAAACCCTGGAAGAATGTCGTGATAGGGGGAAATAGCT29410.11362494880888908No Hit
GTCGTGACTGGGAAAACCCTGGTAGCTAGTCGTGATTGTATCGTGTAGCT29400.11358631400820603No Hit
GTCGTGACTGGGAAAACCCTGGAACGCGGTCGTGATGCCTATAGTTAGCT29360.11343177480547378No Hit
GTCGTGACTGGGAAAACCCTGGAAGTGCGTCGTGATTTGAGCTCGTAGCT29300.1131999660013754No Hit
GTCGTGACTGGGAAAACCCTGGTTCCTTGTCGTGATAACGTGTCGTAGCT29190.11277498319386171No Hit
GTCGTGACTGGGAAAACCCTGGTAAGATGTCGGATGCAACGTAGGTAGCT29090.11238863518703106No Hit
GTCGTGACTGGGAAAACCCTGGCTACGTGTCGTGATGGTCCGCCGTAGCT29060.11227273078498187No Hit
GTCGTGACTGGGAAAACCCTGGCAATTTGTCGTGATATTTACTTCTAGCT29030.1121568263829327No Hit
GTCGTGACTGGGAAAACCCTGGGACATTGTCGTGATCGAGATAGCTAGCT29010.11207955678156656No Hit
GTCGTGACTGGGAAAACCCTGGGAATAGGTCGTGATTTCTCGCGGTAGCT28980.11196365237951737No Hit
GTCGTGACTGGGAAAACCCTGGGGTTGTGTCGTGATGGGAACTCCTAGCT28920.11173184357541899No Hit
GTCGTGACTGGGAAAACCCTGGAACTTTGTCGTGATTCAGAGAGTTAGCT28870.11153866957200369No Hit
GTCGTGACTGGGAAAACCCTGGGCCTGTGTCGTGATATCGATAAATAGCT28820.11134549556858836No Hit
GTCGTGACTGGGAAAACCCTGGCTCTTGGTCGTGATTCTGGAAAGTAGCT28820.11134549556858836No Hit
GTCGTGACTGGGAAAACCCTGGCAAATAGTCGTGATCAATTATCCTAGCT28800.11126822596722223No Hit
GTCGTGACTGGGAAAACCCTGGCAACCCGTCGTGATGCCCCTTCTTAGCT28710.11092051276107466No Hit
GTCGTGACTGGGAAAACCCTGGCCGGGTGTCGTGATCACGTCCAATAGCT28710.11092051276107466No Hit
GTCGTGACTGGGAAAACCCTGGCGCAATGTCGTGATCGAATTGCTTAGCT28600.11049552995356098No Hit
GTCGTGACTGGGAAAACCCTGGAGAGCGGTCGTGATGAATATAGCTAGCT28520.11018645154809648No Hit
GTCGTGACTGGGAAAACCCTGGGGTATGGTCGTGATAAGAATGATTAGCT28390.10968419913921663No Hit
GTCGTGACTGGGAAAACCCTGGAGGTGCGTCGTGATCCATATTCGTAGCT28380.10964556433853358No Hit
GTCGTGACTGGGAAAACCCTGGACCTGCGTCGTGATGATCTGCTGTAGCT28320.10941375553443519No Hit
GTCGTGACTGGGAAAACCCTGGCCTTGCGTCGTGATGTATGCAAATAGCT28290.109297851132386No Hit
GTCGTGACTGGGAAAACCCTGGCATCGGGTCGTGATCGGAAGGTGTAGCT28270.10922058153101989No Hit
GTCGTGACTGGGAAAACCCTGGCCAGGGGTCGTGATAGAATGTCATAGCT28180.10887286832487232No Hit
GTCGTGACTGGGAAAACCCTGGGTGAGCGTCGTGATCGAGTACCCTAGCT28160.10879559872350618No Hit
GTCGTGACTGGGAAAACCCTGGTGTTTAGTCGTGATCTAGCTCCGTAGCT27960.10802290270984492No Hit
GTCGTGACTGGGAAAACCCTGGACTGAGGTCGTGATATGGTACTCTAGCT27940.1079456331084788No Hit
GTCGTGACTGGGAAAACCCTGGGAAAAGGTCGTGATTGCTGCTGCTAGCT27860.1076365547030143No Hit
GTCGTGACTGGGAAAACCCTGGGTAAGAGTCGTGATGCGTACTCTTAGCT27810.10744338069959897No Hit
GTCGTGACTGGGAAAACCCTGGAACCAAGTCGTGATTCAAGCGGCTAGCT27770.10728884149686672No Hit
GTCGTGACTGGGAAAACCCTGGATCCCTGTCGTGATTAGAAGAAATAGCT27700.10701839789208527No Hit
GTCGTGACTGGGAAAACCCTGGCCGGTCGTCGTGATTGCAAGTGCTAGCT27440.10601389307432563No Hit
GTCGTGACTGGGAAAACCCTGGTATTCTGTCGTGATTTAAATTCTTAGCT27250.10527983186134743No Hit
GTCGTGACTGGGAAAACCCTGGTATTTGGTCTGATTTGAATTCCATAGCT27120.1047775794524676No Hit
GTCGTGACTGGGAAAACCCTGGGTGTGAGTCGTGATCATCGATTATAGCT27120.1047775794524676No Hit
GTCGTGACTGGGAAAACCCTGGAAACATGTCGTGATAAAAATGACTAGCT27110.10473894465178454No Hit
GTCGTGACTGGGAAAACCCTGGGCTTTGGTCGTGATCTGGTGAGCTAGCT27090.10466167505041842No Hit
GTCGTGACTGGGAAAACCCTGGTAGCGCGTCGTGATGGCAACTCTTAGCT27030.10442986624632003No Hit
GTCGTGACTGGGAAAACCCTGGCAACTGGTCGTGATAAAATAGTATAGCT26880.10385034423607407No Hit
GTCGTGACTGGGAAAACCCTGGTAAAGTGTCGTGATTTGATGTTATAGCT26880.10385034423607407No Hit
GTCGTGACTGGGAAAACCCTGGTAGTGTGTCGTGATAAGCTTTCTTAGCT26870.10381170943539102No Hit
GTCGTGACTGGGAAAACCCTGGGAACTCGTCGTGATAACTATAACTAGCT26820.10361853543197572No Hit
GTCGTGACTGGGAAAACCCTGGAAGTTCGTCGTGATGCTGACCCCTAGCT26760.10338672662787733No Hit
GTCGTGACTGGGAAAACCCTGGGCGGCTGTCGTGATTATGATCTATAGCT26730.10327082222582815No Hit
GTCGTGACTGGGAAAACCCTGGTCTGATGTCGTGATGATAATTTCTAGCT26710.103193552624462No Hit
GTCGTGACTGGGAAAACCCTGGCAATTAGTCGTGATAACGCAGTCTAGCT26620.10284583941831443No Hit
GTCGTGACTGGGAAAACCCTGGAAAACGGTCGTGATACTCACTCGTAGCT26580.10269130021558219No Hit
GTCGTGACTGGGAAAACCCTGGTGTGAGGTCGTGATAAAAATCCCTAGCT26540.10253676101284993No Hit
GTCGTGACTGGGAAAACCCTGGGTATGCGTCGTGATTTAGCGTCGTAGCT26520.1024594914114838No Hit
GTCGTGACTGGGAAAACCCTGGGCCTTTGTCGTGATTCCCCGTCGTAGCT26470.1022663174080685No Hit
GTCGTGACTGGGAAAACCCTGGGGTTCTGTCGTGATGGAAAACCCTAGCT26460.10222768260738542No Hit
GTCGTGACTGGGAAAACCCTGGGTGCGCGTCGTGATAGGTTATTCTAGCT26440.10215041300601929No Hit
GTCGTGACTGGGAAAACCCTGGTTCCATGTCGTGATTCGTAGTCCTAGCT26390.10195723900260399No Hit
GTCGTGACTGGGAAAACCCTGGATACGCGTCGTGATGGCATATGCTAGCT26340.10176406499918866No Hit
GTCGTGACTGGGAAAACCCTGGAATTCAGTCGTGATTCCGAGTAATAGCT26320.10168679539782255No Hit
GTCGTGACTGGGAAAACCCTGGCTAAGTGTCGTGATTGTCTAAAGTAGCT26320.10168679539782255No Hit
GTCGTGACTGGGAAAACCCTGGCGCGCGGTCGTGATGCGTTCACCTAGCT26290.10157089099577335No Hit
GTCGTGACTGGGAAAACCCTGGTTAACGGTCGTGATGTATTCTGTTAGCT26280.10153225619509028No Hit
GTCGTGACTGGGAAAACCCTGGCTCCATGTCGTGATGAAGTCCCATAGCT26090.1007981949821121No Hit
GTCGTGACTGGGAAAACCCTGGCACTAAGTCGTGATAATTAGTGGTAGCT26040.10060502097869677No Hit
GTCGTGACTGGGAAAACCCTGGGTTGTTGTCGTGATACGGACCCATAGCT26010.10048911657664758No Hit
GTCGTGACTGGGAAAACCCTGGAAGTGCGTCGTGATCTATCTCTATAGCT25970.10033457737391534No Hit
GTCGTGACTGGGAAAACCCTGGTGGAGCGTCGTGATTAGTGCAACTAGCT25960.10029594257323225No Hit
GTCGTGACTGGGAAAACCCTGGGTTGTTGTCGTGACAACTACAAATAGCT25950.1002573077725492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)