FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016985
Sequences flagged as poor quality0
Sequence length248
%GC67

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGATTCCTGTCGTGATGGAAATGAGATCAC32550.3200637177539492No Hit
GTCGTGACTGGGAAAACCCTGGTGTATGGTCGTGATGGATACCAGTACAC19130.18810503596414893No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTCGTGATGCCGCGAGATCAC19020.18702340742488827No Hit
GTCGTGACTGGGAAAACCCTGGGTCCGTGTCGTGATAAGAGGCCCTGCAC17500.17207726760965011No Hit
GTCGTGACTGGGAAAACCCTGGTTGACAGTCGTGATCGAGTTGACATCAC10670.1049179683082838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC903300.0242.30522
CGTGACT890850.0242.036583
GTGACTG928650.0242.019184
TGACTGG928550.0241.781545
GACTGGG937200.0241.687046
ACTGGGA932600.0241.456737
TGGGAAA902450.0240.512549
CTGGGAA939200.0238.87198
GGTGACT39050.0237.721533
GTGAGTG9650.0236.01614
GTGACAG12500.0235.508934
TCGGGAC6750.0233.537772
TGGTGAC47150.0232.747182
GTGGTGA51400.0232.219851
TCGTGGC3250.0231.326742
CGTGACA11800.0230.052433
AGACTGG950.0229.439215
CGTGACG10400.0227.22813
GACGGGG20350.0225.46896
TGACAGG13050.0224.555445
Produced by FastQC (version 0.11.5)