FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697792
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGACCACTGTCGTGATATTAACTATCGATG46860.6715468219756031No Hit
GTCGTGACTGGGAAAACCCTGGCTGTGGGTCGTGATAGCTGTGGACGATG8110.11622374575804824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA665650.0241.027397
TCGTGAC667550.0240.7982
TGGGAAA634100.0239.853479
CGTGACT667950.0239.806693
GTGACTG678500.0237.831424
TGACTGG680200.0237.293565
GACTGGG681900.0236.792286
CTGGGAA680700.0236.161518
CGTGGCT8950.0213.833883
CGTGCCT451.8189894E-12188.420413
TGGTGAC10550.0173.441862
GTGACTT3750.0171.132024
TGGGAAC31900.0170.330839
CGTGACA2500.0164.733293
GTGGTGA11300.0160.938691
TGACAGG2750.0158.464485
GTGACAG2600.0158.340584
GGTGACT11350.0155.811483
CTGCGAA557.2759576E-12154.029568
TCGTGGC11350.0147.337282
Produced by FastQC (version 0.11.5)