FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19790
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGACGGAGGTCGTGATTCTGGCAAAGATCA13887.013643254168772No Hit
GTCGTGACTGGGAAAACCCTGGGCTTTTAGTCGTGATTAAAGCGAGATCA9814.957049014653866No Hit
GTCGTGACTGGGAAAACCCTGGAATGTCGTCGTGATGAAACGTCTGATCA4702.374936836786256No Hit
GTCGTGACTGGGAAAACCCTGGTGCTCAGTCGTGATTGGGGCAGAGATCA3341.687721071248105No Hit
GTCGTGACTGGGAAAACCCTGGGATCCTGTCGTGATATGGGTGATGATCA3161.5967660434562911No Hit
GTCGTGACTGGGAAAACCCTGGCGACCAGTCGTGATGACAATTACGTTCA3101.5664477008590199No Hit
GTCGTGACTGGGAAAACCCTGGTCTTTTGTCGTGATCCTAAGAGCGATCA2781.4047498736735724No Hit
GTCGTGACTGGGAAAACCCTGGGATGGGGTCGTGATAGATGATCGGATCA2361.192521475492673No Hit
GTCGTGACTGGGAAAACCCTGGTGCGCGGTCGTGATATCAACCCCGATCA1900.9600808489135927No Hit
GTCGTGACTGGGAAAACCCTGGGAATAGGTCGTGATATGGATTGGGATCA1760.8893380495199597No Hit
GTCGTGACTGGGAAAACCCTGGCCCGAGTCGTGATGTAACAGACAGATCA1550.7832238504295099No Hit
GTCGTGACTGGGAAAACCCTGGCGGACCGTCGTGATGTAAGGTTCCAGCA1500.7579585649317837No Hit
GTCGTGACTGGGAAAACCCTGGGCAGCAGTCGTGATTCGAAGCCCGATCA1080.5457301667508843No Hit
GTCGTGACTGGGAAAACCCTGGCCTAAAGTCGTGATCCTTTACTCTCTCA970.4901465386558868No Hit
GTCGTGACTGGGAAAACCCTGGCGCGCTGTCGTGATATAAGGTGCGATCA900.4547751389590703No Hit
GTCGTGACTGGGAAAACCCTGGTCGCAAGTCGTGATAATTGACCTTGATA870.43961596766043454No Hit
GTCGTGACTGGGAAAACCCTGGGCCTCAGTCGTGATTCAAATTAGGATCA700.35371399696816574No Hit
GTCGTGACTGGGAAAACCCTGGGTGAACGTGGCCGGCCGGGCGCGGCTCA700.35371399696816574No Hit
GTCGTGACTGGGAAAACCCTGGCTGTCTGTCGTGATGTATAGAACGATCA660.33350176856998487No Hit
GTCGTGACTGGGAAAACCCTGGACGCGTGTCGTGATGCAATGGATGATCA650.32844871147043964No Hit
GTCGTGACTGGGAAAACCCTGGACCCCGGTCGTGATGACAACTCAGATCA600.30318342597271347No Hit
GTCGTGACTGGGAAAACCCTGGGCGACCGTCGTGATATGTGGTGCGATCA570.2880242546740778No Hit
GTCGTGACTGGGAAAACCCTGGTCTCCCGTCGTGATTGGCAGGGTGATCA530.2678120262758969No Hit
GTCGTGACTGGGAAAACCCTGGTCGCCCGTCGTGATGGCCTGGCTGATCA460.23244062657908038No Hit
GTCGTGACTGGGAAAACCCTGGGCTAACGTCGTGATTCCGACTAAGATCA440.2223345123799899No Hit
GTCGTGACTGGGAAAACCCTGGCCCCCCGTCGTGATCCCTATGAGGATCA430.21728145528044468No Hit
GTCGTGACTGGGAAAACCCTGGAACCGGGTCGTGATCTCACACCCGATCA430.21728145528044468No Hit
GTCGTGACTGGGAAAACCCTGGACCCATGTCGTGATAAGTGTCACGGTCA430.21728145528044468No Hit
GTCGTGACTGGGAAAACCCTGGCGCCTTGTCGTGATTGTGAGGGGTGATA420.21222839818089945No Hit
GTCGTGACTGGGAAACCCCTGGGCTTTTAGTCGTGATTAAAGCGAGATCA420.21222839818089945No Hit
GTCGTGACTGGGAAAACCCTGGGGGAAAGTCGTGATATTACGGATGATCA400.202122283981809No Hit
GTCGTGACTGGGAAAACCCTGGGAACGCGTCGTGATTGTCATTGGGATCA390.19706922688226378No Hit
GTCGTGACTGGGAAAACCCTGGGAATTAGTCGTGATCTAAAGGAGGATCA380.19201616978271854No Hit
GTCGTGACTGGGAAAACCCTGGACGTGGTCGTGATCTGGACAGCTGATCA380.19201616978271854No Hit
GTCGTGACTGGGAAAACCCTGGATCCAGGTCGTGATAAGAGCCCCGATCA370.1869631126831733No Hit
GTCGTGACTGGGAAAACCCTGGCAAGCCGTCGTGATTGTTAACCCGATCA370.1869631126831733No Hit
GTCGTGACTGGGAAAACCCTGGTCTTGCTACTCATACGTGAAAATGATCA360.1819100555836281No Hit
GTCGTGACTGGGAAAACCCTGGACCCGGGTCGTGATGTGATGTTGTCTCA340.17180394138453764No Hit
GTCGTGACTGGGAAAACCCTGGTAAGGCGTCGTGATGAGTGACCTGATCA340.17180394138453764No Hit
GTCGTGACTGGGAAAACCCTGGTCGGAAGTCGTGATCCGTGGGCAGATCA340.17180394138453764No Hit
GTCGTGACTGGGAAAACCCTGGAAGGGAGTCGTGATAACTCGGAGGATCA340.17180394138453764No Hit
GTCGTGACTGGGAAAACCCTGGCGAATCGTCGTGATTCCAAGGGCGATCA330.16675088428499243No Hit
GTCGTGACTGGGAAAACCCTGGATCCCGGTCGTGATTGAGAACAGGATCA330.16675088428499243No Hit
GTCGTGACTGGGAAAACCCTGGTGTCGTGTCGTGATTACCTCACCGATCA320.1616978271854472No Hit
GTCGTGACTGGGAAAACCCTGGTGGGCTGTCGTGATAGTGAAGATGATCA310.15664477008590197No Hit
GTCGTGACTGGGAAAACCCTGGGACATCGTCGTGATGTGAAAGCTGATCA300.15159171298635674No Hit
GTCGTGACTGGGAAAACCCTGGCAAACGTCGTGATCACTACCCCAGATCA300.15159171298635674No Hit
GTCGTGACTGGGAAAACCCTGGTATTATGTCGTGATATGCAGGGAGATCA300.15159171298635674No Hit
GTCGTGACTGGGAAAACCCTGGAATTCTGTCGTGATCCTATTACCGATCA300.15159171298635674No Hit
GTCGTGACTGGGAAAACCCTGGTTCGAGGTCGTGATCCATAAGGGGATCA300.15159171298635674No Hit
GTCGTGACTGGGAAAACCCTGGGGACGCGTCGTGATGACCGGGGGGATCA290.1465386558868115No Hit
GTCGTGACTGGGAAAACCCTGGTCTTCAGTCGTGATTCTGCGTAGGATCA290.1465386558868115No Hit
GTCGTGACTGGGAAAACCCTGGATCTAGGTCGTGATACAAATGGAGATCA290.1465386558868115No Hit
GTCGTGACTGGGAAAACCCTGGGTTTGTGTCGTGATATCCTTCGGGATCA290.1465386558868115No Hit
GTCGTGACTGGGAAAACCCTGGAAATATGTCGTGATCTTATTGGGGATCA280.1414855987872663No Hit
GTCGTGACTGGGAAAACCCTGGCTCAATGTCGTGATTAAACCACTGATCG280.1414855987872663No Hit
GTCGTGACTGGGAAAACCCTGGTACCAGGTCGTGATTCTAAGATCGATCA270.13643254168772107No Hit
GTCGTGACTGGGAAAACCCTGGGCCACCGTCGTGATGCGTTAATAGATCA270.13643254168772107No Hit
GTCGTGACTGGGAAAACCCTGGAATCAAGTCGTGATGTTTACACTGATCG260.13137948458817583No Hit
GTCGTGACTGGGAAAACCCTGGCCTTTTGTCGTGATGGTGTCACCGATCA260.13137948458817583No Hit
GTCGTGACTGGGAAAACCCTGGCGGTCCGTCGTGATTTCATGCAAGATCA250.12632642748863063No Hit
GTCGTGACTGGGAAAACCCTGGTTATTCGTCGTGATAGGAACTCCGATCA240.1212733703890854No Hit
GTCGTGACTGGGAAAACCCTGGCATTAGGTCGTGATCGGAAAACTGATCA240.1212733703890854No Hit
GTCGTGACTGGGAAAACCCTGGACCCCCGTCGTGATCAAAGACACGATCA240.1212733703890854No Hit
GTCGTGACTGGGAAAACCCTGGACAAATGTCGTGATTACCCGTTCGATCA220.11116725618999496No Hit
GTCGTGACTGGGAAAACCCTGGATATCCGTCGTGATGTAAAGGAGGATCA220.11116725618999496No Hit
GTCGTGACTGGGAAAACCCTGGCCAAACGTCGTGATACCTGGGAAGATCA220.11116725618999496No Hit
GTCGTGACTGGGAAAACCCTGGCGCCCGGTCGTGAACACTGCCAAGATCA210.10611419909044972No Hit
GTCGTGACTGGGAAAACCCTGGGTGAAGGTCGTGATTACGTAGCCGATCA210.10611419909044972No Hit
GTCGTGACTGGGAAAACCCTGGCAGGTAGTCGTGATTACTGCAAAGATCA210.10611419909044972No Hit
GTCGTGACTGGGAAAACCCTGGCCCTTCGTCGTGATATCAAGGACGATCA210.10611419909044972No Hit
GTCGTGACTGGGAACACCCTGGGCTTTTAGTCGTGATTAAAGCGAGATCA210.10611419909044972No Hit
GTCGTGACTGGGAAAACCCTGGGAAGAAGTCGTGATCAAAGTGATGATCA210.10611419909044972No Hit
GTCGTGACTGGGAAAACCCTGGTGATAGGTCTGATTACCCTATCCGATCA210.10611419909044972No Hit
GTCGTGACTGGGAAAACCCTGGCATCCATCGTGATCTCAAAGGGCGATCA200.1010611419909045No Hit
GTCGTGACTGGGAAAACCCTGGTGGAGGGTCGTGATGGCCGCTATGATCA200.1010611419909045No Hit
GTCGTGACTGGGAAAACCCTGGTTTTCAGTCGTGATTGCGGGGGGGATCA200.1010611419909045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCT1900.0242.000023
TCGTGGC1400.0242.000022
TCGTGAC14950.0242.02
TGACTGG14900.0241.187915
CGTGACT15050.0239.588033
GACTGGG15000.0239.586
GTGACTG15100.0238.794714
ACTGGGA15000.0236.353337
TGGGAAA15700.0229.66889
CTGGGAA16400.0225.768288
GGCGTGG650.0223.384631
TGGCTGG2150.0213.860475
GTGGCTG2200.0209.04
CTGGGGG1350.0206.148158
GTCGTGG1650.0205.333331
ACTGGGG254.927133E-7193.599997
TGGGGGA1100.0187.09
GGCTGGG2850.0182.56146
GCGGGGC401.2914825E-10181.499982
TGGGGGG600.0161.333339
Produced by FastQC (version 0.11.5)