FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151162
Sequences flagged as poor quality0
Sequence length248
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTTCGAAGTCGTGATTGACAGGCTACAGT6010.39758669506886646No Hit
GTCGTGACTGGGAAAACCCTGGGAGCAGGTCGTGATGCATAGTCAACAGT2760.18258557044759927No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTCACTGGGGGGGAGACAGTG1810.11973908786599807No Hit
GTCGTGACTGGGAAAACCCTGGTAACCGGTCGTGATATAAAGTGTCAGTG1760.11643137825644011No Hit
GTCGTGACTGGGAAAACCCTGGACGGTAGTCGTGATAATGATTGAACAGT1570.10386208174011986No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC100.0015218452242.451132
TCGGACT151.5813957E-5242.45112
TCGTACT151.5813957E-5242.45112
CTACGCT100.0015248685242.29053
TACCCAA100.0015278958242.130089
TCGTGAC137250.0241.126252
ACTGGGA134200.0239.784567
CGTGACT137350.0239.732653
TGGGAAA131650.0238.267789
CTGGGAA136100.0236.437098
CGTGGCT1500.0234.214143
TGACTGG140300.0232.715215
GTGACTG141450.0232.44134
GACTGGG139550.0231.285866
GCGCCAC1050.0219.360532
TCGTGGC1450.0217.369952
GTGACTT650.0186.37734
GCCACTG1300.0177.058444
ACTGCCC1200.0171.50887
CGCCACT1400.0164.41143
Produced by FastQC (version 0.11.5)