FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049865
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG1966650.0241.312874
TGACTGG1966750.0241.27735
CGTGACT1968000.0241.16823
TCGTGAC1977650.0240.716422
GACTGGG1972250.0240.353366
ACTGGGA1963750.0240.038337
TGGGAAA1982400.0233.091589
CTGGGAA2059350.0229.923698
CGTGGCT31800.0224.684233
TCGTGGC28700.0213.16522
GCGTGGC7750.0184.45362
GTCGTGG37700.0165.591481
GTGGCTG49700.0147.853524
GTGGTGA14100.0134.115051
ACTTGGA12750.0133.84837
CGTGACA9200.0132.947853
GTCGTGA3843400.0123.89711
GGCGTGG12300.0118.262811
TTGGAAA15050.0117.411259
TGACCGG2700.0116.561715
Produced by FastQC (version 0.11.5)