FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361442
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCGTTTTGTCGTGATCGTCGGAGCGCCAG122360.5181579729673649No Hit
GTCGTGACTGGGAAAACCCTGGTATCAGATCAAAACCAACCCGGTGGTAA63720.2698351261644368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCCAA29670.12564356863306403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT2321250.0241.321233
TGACTGG2323150.0241.105795
TCGTGAC2326200.0241.086462
GTGACTG2324100.0241.047364
ACTGGGA2331550.0239.936757
GACTGGG2335650.0239.62916
CTGGGAA2402300.0232.875558
TGGGAAA2437450.0229.497429
ACGTGAC3200.0177.935182
ACCGGGA5250.0145.201357
CGTGGCT6550.0133.101883
CGAGACT6000.0131.176223
GTCGTGA4532350.0123.68431
TCGAGAC6050.0116.1412662
TGCGAAA3450.0112.230559
TGACCGG8100.0109.064265
GACGTGA5400.0100.9989551
TAGTGAC3150.099.994642
TCGTGGC8750.099.686962
CGTGACC9100.091.812273
Produced by FastQC (version 0.11.5)