FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152477
Sequences flagged as poor quality0
Sequence length248
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGGTTGGGTCGTGATGTGGATTTCCAGAT13920.9129245722305659No Hit
GTCGTGACTGGGAAAACCCTGGTGGTCCGTCGTGATTTGATAGTGCAGAT11530.7561796205329329No Hit
GTCGTGACTGGGAAAACCCTGGGCTTTCGTCGTGATTGGGAATACCAGAT9800.6427198856220938No Hit
GTCGTGACTGGGAAAACCCTGGGCTAGGGTCGTGATATCTGGAGACAGAT8980.5889412829475921No Hit
GTCGTGACTGGGAAAACCCTGGGTAGTGGTCGTGATTTTGAGAAGCAGAT8130.5331951704191452No Hit
GTCGTGACTGGGAAAACCCTGGGGACGAGTCGTGATACGCCGATTCAGAT7300.4787607311266618No Hit
GTCGTGACTGGGAAAACCCTGGCCCATCGTCGTGATACTAAGTAACAGAT4590.30102900765361335No Hit
GTCGTGACTGGGAAAACCCTGGGTTCAGGTCGTGATACGTACATTCAGAT3720.24397122188920295No Hit
GTCGTGACTGGGAAAACCCTGGAATCTTGTCGTGATTGTATGTACCAGAT3620.2374128557093857No Hit
GTCGTGACTGGGAAAACCCTGGAAGCCTGTCGTGATGACCTCGGACAGAT3220.21117939099011657No Hit
GTCGTGACTGGGAAAACCCTGGAATTACGTCGTGATCATACAGAACAGAT2530.16592666434937728No Hit
GTCGTGACTGGGAAAACCCTGGTAATTCGTCGTGATGCTAAGAGCCAGAT2220.14559572919194372No Hit
GTCGTGACTGGGAAAACCCTGGTCCAAGGTCGTGAGTGTGAGGATCAGAT2190.14362821933799852No Hit
GTCGTGACTGGGAAAACCCTGGGAACGCGTCGTGATGTCGACGGCCAGAT2100.13772568977616298No Hit
GTCGTGACTGGGAAAACCCTGGTCGGTGGTCGTGATGCCCGCCCTCAGAT2090.13706985315818124No Hit
GTCGTGACTGGGAAAACCCTGGTTCTCTGTCTCTGAAGGAACCCCCAGAT2080.1364140165401995No Hit
GTCGTGACTGGGAAAACCCTGGGATCCTGTCGTGATCTAGCATGGCAGAT2000.1311673235963457No Hit
GTCGTGACTGGGAAAACCCTGGCCTCAGGTCGTGATACTTACCCTCAGAT1970.12919981374240047No Hit
GTCGTGACTGGGAAAACCCTGGTTGATGGTCGTGATTACCTCACGCAGAT1940.1272323038884553No Hit
GTCGTGACTGGGAAAACCCTGGGCGTTGGTCGTGATAGTTTTGCCCAGAT1920.12592063065249184No Hit
GTCGTGACTGGGAAAACCCTGGTGGATGGTCGTGATTGACCGGGCCAGAT1820.11936226447267456No Hit
GTCGTGACTGGGAAAACCCTGGGCGTTGGTCGTGATAAATGGCTGCAGAT1810.11870642785469285No Hit
GTCGTGACTGGGAAAACCCTGGCGGCGAGTCGTGTTATCGCCTGACAGAT1700.11149222505689382No Hit
GTCGTGACTGGGAAAACCCTGGCTGCCTGTCGTGATGTCCGGAAACAGAT1690.1108363884389121No Hit
GTCGTGACTGGGAAAACCCTGGGCTCCCGTCGTGATATCAGTGGCCAGAT1620.10624553211304No Hit
GTCGTGACTGGGAAAACCCTGGCAGGGAGTCGTGATTACCGGATGCAGAT1620.10624553211304No Hit
GTCGTGACTGGGAAAACCCTGGGCAGTCGTCGTGATTTAACAAGACAGAT1610.10558969549505828No Hit
GTCGTGACTGGGAAAACCCTGGAATCTCGTCGTGATGGTGAGAAACAGAT1580.1036221856411131No Hit
GTCGTGACTGGGAAAACCCTGGGGAGAGGTCGTGATTAACTGGCTCAGAT1580.1036221856411131No Hit
GTCGTGACTGGGAAAACCCTGGATCAAGGTCGTGATTCGCTTGTACAGAT1580.1036221856411131No Hit
GTCGTGACTGGGAAAACCCTGGCGTGTGGTCGTGATCACTTGTCACAGAT1550.1016546757871679No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGT100.0015209704242.498352
GTGCTAT151.5801925E-5242.498341
GCACGTG100.0015224682242.418663
TTGATCG2600.0242.179927
TTATCGG1200.0242.17998
CGATATC201.6498961E-7242.179896
TCGTGAC108950.0241.496752
TGACTGG109500.0241.374485
GTGACTG109750.0241.124574
ACTGGGA110000.0240.198437
CGTGACT107800.0240.169893
GACTGGG110800.0240.103446
TGGGAAA110100.0236.020949
ATGATCG5950.0234.03947
CTGGGAA112500.0233.891978
TATCGGA1900.0229.433589
ACGGGGA1850.0229.08917
AAGATCG7150.0226.93787
TGATCGG12600.0222.959298
ACGAGAT600.0222.071145
Produced by FastQC (version 0.11.5)