FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551552
Sequences flagged as poor quality0
Sequence length248
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGGTCTTTGTCGTGATTACGAGCGGTGACC457252.94704914820773No Hit
GTCGTGACTGGGAAAACCCTGGGAAAAGGTCGTGATGCCCTGTGTTGACC402422.593661056799901No Hit
GTCGTGACTGGGAAAACCCTGGATAGCTGTCGTGATGCGAGTACCTGACC388312.5027198572783895No Hit
GTCGTGACTGGGAAAACCCTGGCACGTAGTCGTGATTAACTAAGGTGACC381142.4565080641834753No Hit
GTCGTGACTGGGAAAACCCTGGACTGTGGTCGTGATTCAATGTACTGACC329462.123422224972157No Hit
GTCGTGACTGGGAAAACCCTGGCTCCTCGTCGTGATCGCCGTAGGTGACC310221.9994173575877572No Hit
GTCGTGACTGGGAAAACCCTGGGATCACGTCGTGATATGAACTCCTGACC268711.731878789753743No Hit
GTCGTGACTGGGAAAACCCTGGCACACAGTCGTGATGATGAGGAATGACC259731.6740012580951202No Hit
GTCGTGACTGGGAAAACCCTGGCGCCGAGTCGTGATGAGGACCCGTGACC253871.6362326238501836No Hit
GTCGTGACTGGGAAAACCCTGGCAGATAGTCGTGATCTCGACCCGTGACC205301.3231912304582765No Hit
GTCGTGACTGGGAAAACCCTGGTAAAGTGTCGTGATTAACATGCGTGACC203821.3136523945056304No Hit
GTCGTGACTGGGAAAACCCTGGACGCCCGTCGTGATGGACCCTCTTGACC175031.1280962545889535No Hit
GTCGTGACTGGGAAAACCCTGGCGTATAGTCGTGATCCAGAGGATTGACC170591.0994797467310151No Hit
GTCGTGACTGGGAAAACCCTGGAATGAAGTCGTGATCCATATAACTGACC152660.9839180381965928No Hit
GTCGTGACTGGGAAAACCCTGGCCTAATGTCGTGATGCGTACTCTTGACC150020.9669028173080889No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGGGTCGTGATCCTGGTGGCTGACC140700.9068339314441282No Hit
GTCGTGACTGGGAAAACCCTGGAAGTATGTCGTGATTAGCTCGAATGACC124700.8037113806047106No Hit
GTCGTGACTGGGAAAACCCTGGAAACCAGTCGTGATACATAGGGGTGACC120030.7736124860784556No Hit
GTCGTGACTGGGAAAACCCTGGCAATGGGTCGTGATATTGAGTTCTGACC99240.6396176215814875No Hit
GTCGTGACTGGGAAAACCCTGGTAAAACGTCGTGATGTATACTAGTGACC94920.6117745328548447No Hit
GTCGTGACTGGGAAAACCCTGGACCTTCGTCGTGATAACTACGGCTGACC93570.6030735676277688No Hit
GTCGTGACTGGGAAAACCCTGGATTCCTGTCGTGATGGAAATGAGTGACC88180.56833415831374No Hit
GTCGTGACTGGGAAAACCCTGGATTAGTGTCGTGATCGAAAGCCTTGACC83470.5379774574103865No Hit
GTCGTGACTGGGAAAACCCTGGGCCTCCGTCGTGATGATAAAAGCTGACC83330.5370751350905416No Hit
GTCGTGACTGGGAAAACCCTGGTCTCAGGTCGTGATGTGGAGGGATGACC81870.5276652023264448No Hit
GTCGTGACTGGGAAAACCCTGGCCCTAGGTCGTGATAAGGCCTTGTGACC78790.5078141112898569No Hit
GTCGTGACTGGGAAAACCCTGGGATGACAGTCGTGATTAGCACCCTGACC77950.5024001773707875No Hit
GTCGTGACTGGGAAAACCCTGGTAACTCGTCGTGATCATAGGGATTGACC77510.49956430722270345No Hit
GTCGTGACTGGGAAAACCCTGGTATCTAGTCGTGATTTAGTGTAGTGACC77480.4993709524398796No Hit
GTCGTGACTGGGAAAACCCTGGTTGTAAGTCGTGATTGTACACGCTGACC74570.4806155385059605No Hit
GTCGTGACTGGGAAAACCCTGGAAAGGCGTCGTGATCTCGGTGCCTGACC74340.4791331518376438No Hit
GTCGTGACTGGGAAAACCCTGGAGCGTAGTCGTGATCGATATGCCTGACC71390.4601199315266262No Hit
GTCGTGACTGGGAAAACCCTGGAAACCAGTCGTGATGCCAAGGGGTGACC71170.45870199645258425No Hit
GTCGTGACTGGGAAAACCCTGGCTAATAGTCGTGATAATGGCAGCTGACC69350.44697180629460054No Hit
GTCGTGACTGGGAAAACCCTGGCAAGTTGTCGTGATAATGGATGATGACC68550.4418156787526296No Hit
GTCGTGACTGGGAAAACCCGGGGTCTTTGTCGTGATTACGAGCGGTGACC66080.4258961349667945No Hit
GTCGTGACTGGGAAAACCCTGGGGCCGTGTCGTGATTACCTGGCTTGACC64560.4160994926370499No Hit
GTCGTGACTGGGAAAACCCTGGTTGTTGGTCGTGATGGAGTGGGCTGACC57500.3705966670791569No Hit
GTCGTGACTGGGAAAACCCTGGGTAGTCGTCGTGATAAGGAGGGCTGACC56280.36273357257765126No Hit
GTCGTGACTGGGAAAACCCGGGATAGCTGTCGTGATGCGAGTACCTGACC54900.3538392525677515No Hit
GTCGTGACTGGGAAAACCCTGGCAGCCAGTCGTGATGCCTACTAATGACC54310.350036608505548No Hit
GTCGTGACTGGGAAAACCCTGGCCAAAAGTCGTGATAGCCGAGGATGACC53470.3446226745864786No Hit
GTCGTGACTGGGAAAACCCTGGAGCTCCGTCGTGATACCCCCGGGTGACC53370.3439781586437322No Hit
GTCGTGACTGGGAAAACCCTGGTGATTGGTCGTGATCAGTAGTTGTGACC52990.34152899806129605No Hit
GTCGTGACTGGGAAAACCCTGGTTCTAAGTCGTGATTTGCGCCGGTGACC52000.3351482902281071No Hit
GTCGTGACTGGGAAAACCCTGGGGCAACGTCGTGATAAGGATTCCTGACC50400.3248360351441653No Hit
GTCGTGACTGGGAAAACCCTGGCTCATCGTCGTGATTCAGCGTGGTGACC49270.3175530049911314No Hit
GTCGTGACTGGGAAAACCCTGGGCTTTCGTCTGATACTTTCGCCCTGACC47620.3069184919358165No Hit
GTCGTGACTGGGAAAACCCTGGCACAGTGTCGTGATTATATCTCATGACC45030.2902255290186858No Hit
GTCGTGACTGGGAAAACCCTGGACTTATGTCGTGACCTTCGTGAATGACC44970.289838819453038No Hit
GTCGTGACTGGGAAAACCCTGGCTCATCGTCGTGATACATGTGGCTGACC44600.28745411046487646No Hit
GTCGTGACTGGGAAAACCCTGGTCGACAGTCGTGATGTTAAAGAATGACC44060.2839737243740461No Hit
GTCGTGACTGGGAAAACCCTGGAGCCTCGTCGTGATCTAAAGGAGTGACC43330.2792687579919977No Hit
GTCGTGACTGGGAAAACCCTGGCTTACAGTCGTGATCAAGAACCTTGACC42690.275143855958421No Hit
GTCGTGACTGGGAAAACCCTGGTTCCCTGTCGTGTCGCACACCCTTGACC42400.2732747597244566No Hit
GTCGTGACTGGGAAAACCCTGGTTCAGCGTCGTGATTTAAATGAGTGACC42270.27243688899888624No Hit
GTCGTGACTGGGAAAACCCTGGCTTAGTGTCGTGATCGGAAGTAGTGACC41570.26792527739966177No Hit
GTCGTGACTGGGAAAACCCTGGCGGGGAGTCGTGATGCGCCTCCTTGACC40830.2631558594233387No Hit
GTCGTGACTGGGAAAACCCTGGGGCGCGGTCGTGATATTGTTGGCTGACC40150.2587731510126634No Hit
GTCGTGACTGGGAAAACCCTGGGTGTCAGTCGTGATGATGGTGGCTGACC39890.2570974095615229No Hit
GTCGTGACTGGGAAAACCCTGGACGCCCGTCGTGATAGAGAGGCTTGACC39110.2520701852081013No Hit
GTCGTGACTGGGAAAACCCTGGGTCGTGGTCGTGATCCCAGCTTGTGACC38770.24987883100276367No Hit
GTCGTGACTGGGAAAACCCTGGATAGCCGTCGTGATGCAATTTGATGACC38340.24710741244895434No Hit
GTCGTGACTGGGAAAACCCTGGGATTTCGTCGTGATACGTATGCCTGACC37780.24349812316957473No Hit
GTCGTGACTGGGAAAACCCTGGCGATGCGTCGTGATTAAGTGACCTGACC37720.2431114136039269No Hit
GTCGTGACTGGGAAAACCCTGGACTAGTGTCGTGATAAACGGTGCTGACC37010.23853535041042775No Hit
GTCGTGACTGGGAAAACCCTGGAACAAGGTCGTGATTTAAGGACTTGACC36860.23756857649630822No Hit
GTCGTGACTGGGAAAACCCTGGCCCGCAGTCGTGATAGAATATAGTGACC36620.23602173823371697No Hit
GTCGTGACTGGGAAAACCCTGGGCAGTCGTCGTGATGAGATACGCTGACC36390.23453935156540034No Hit
GTCGTGACTGGGAAAACCCTGGAAACTCGTCGTGATATTAAACAGTGACC36110.23273470692571052No Hit
GTCGTGACTGGGAAAACCCGGGGAAAAGGTCGTGATGCCCTGTGTTGACC34740.22390483851008539No Hit
GTCGTGACTGGGAAAACCCTGGCCCTAGGTCGTGATAAGGCGTTGTGACC34540.22261580662459265No Hit
GTCGTGACTGGGAAAACCCTGGACATGAGTCGTGATGATAACCGCTGACC33600.21655735676277688No Hit
GTGGTGACTGGGAAAACCCTGGACTGTGGTCGTGATTCAATGTACTGACC33540.21617064719712908No Hit
GTCGTGACTGGGAAAACCCTGGTATTTAGTCGTGATTGTGTGGCTTGACC33090.21327032545477045No Hit
GTCGTGACTGGGAAAACCCTGGCCTAGGGTCGTGATGGTTGTCCCTGGTC33050.2130125190776719No Hit
GTCGTGACTGGGAAAACCCTGGTACGGTGTCGTGATTGGTAATAGTGACC32610.2101766489295879No Hit
GTCGTGACTGGGAAAACCCTGGCTTTGGGTCGTGATTAGACCAGGTGACC30830.1987042651487027No Hit
GTCGTGACTGGGAAAACCCTGGAGAGGAGTCGTGATGGAAATTGGTGACC30760.19825310398878027No Hit
GTCGTGACTGGGAAAACCCTGGCAGATGGTCGTGATCGCAGGACATGACC30420.19606174978344265No Hit
GTCGTGACTGGGAAAACCCTGGTTTCCTGTCGTGATGGCATGTTTTGACC30280.19515942746359774No Hit
GTCGTGACTGGGAAAACCCTGGGTATCGGTCGTGATATGGTCAGCTGACC29160.1879408489048385No Hit
GTCGTGACTGGGAAAACCCGGGTAAAGTGTCGTGATTAACATGCGTGACC27260.17569504599265767No Hit
GTCGTGACTGGGAAAACCCTGGGGCTACGTCGTGATTACCAACCGTGGTC26750.17240801468465125No Hit
GTCGTGACTGGGAAAACCCTGGTGCGTCGTCGTGATGGGAACGCCTGACC26370.1699588541022151No Hit
GTCGTGACTGGGAAAACCCTGGATCAAAGTCGTGATAGTAATGATTGACC26340.16976549931939117No Hit
GTGGTGACTGGGAAAACCCTGGGTCTTTGTCGTGATTACGAGCGGTGACC25520.164480468588871No Hit
GTCGTGACTGGGAAAACCCTGGGAGAGTGTCGTGATGGGCATCGTTGACC25320.1631914367033783No Hit
GTCGTGACTGGGAAAACCCTGGCACGTAGTCGTGATTAACTAAGGTGACG24720.15932434104690013No Hit
GTCGTGACTGGGAAAACCCTGGGTCATTGTCGTGATCTAAAAACTTGACC24400.15726189003011176No Hit
GTCGTGACTGGGAAAACCCTGGTAAAACGTCGTGATGTATACTAGTGGAC23820.1535236975621829No Hit
GTCGTGACTGGGAAAACCCTGGCCCAAAGTCGTGATTCTGACCCGTGACC23750.15307253640226046No Hit
GTCGTGACTGGGAAAACCCTGGCTAGACGTCGTGATCGTGTGTGCTGACC23700.15275027843088726No Hit
GTCGTGACTGGGAAAACCCTGGCCTCGAGTCGTGATTTAGGTGGGTGACC23040.14849647320876128No Hit
GTCGTGACTGGGAAAACCCGGGAAGTATGTCGTGATTAGCTCGAATGACC22780.14682073175762075No Hit
GTCGTGACTGGGAAAACCCTGGCGCTATGTCGTGATCGTTATGCCTGACC22760.14669182856907148No Hit
GTCGTGACTGGGAAAACCCTGGCGGTTGGTCGTGATGTCCTGGGATGACC22190.14301808769541724No Hit
GTGGTGACTGGGAAAACCCGGGGTCTTTGTCGTGATTACGAGCGGTGACC22070.1422446685641216No Hit
GTCGTGACTGGGAAAACCCTGGATCGCCGTCGTGATCAAAAGTTGTGACC22010.14185795899847378No Hit
GTCGTGACTGGGAAAACCCTGGGCCTAGGTCGTGATTTGGAGGTGTGACC20970.13515499319391164No Hit
GTCGTGACTGGGAAAACCCGGGCACGTAGTCGTGATTAACTAAGGTGACC20920.13483273522253847No Hit
GTCGTGACTGGGAAAACCCGGGCGCCGAGTCGTGATGAGGACCCGTGACC20860.13444602565689065No Hit
GTCGTGACTGGGAAAACCCTGGGTAGCTGGCGTGATGAGAGCCCGTGACA20780.13393041290269356No Hit
GTCGTGACTGGGAAAACCCGGGCGTATAGTCGTGATCCAGAGGATTGACC20770.1338659613084189No Hit
GTGGTGACTGGGAAAACCCTGGCTCCTCGTCGTGATCGCCGTAGGTGACC20370.13128789753743347No Hit
GTCGTGACTGGGAAAACCCTGGTCCAGAGTCGTGGATTGACTCCATGACC19330.12458493173287134No Hit
GTCGTGACTGGGAAAACCCGGGGATCACGTCGTGATATGAACTCCTGACC18250.11762415955121067No Hit
GTCGTGACTGGGAAAACCCTGGCTATCCGTCGTGATCTATTTACATGACC18120.11678628882564039No Hit
GTCGTGACTGGGAAAACCCTGGTACCCAGTCGTGATCGCTAACAATGACC18070.11646403085426721No Hit
GTCGTGACTGGGAAAACCCTGGGCACTGTCCTGATCGTACGGGAATGACC18040.1162706760714433No Hit
GTCGTGACTGGGAAAACCCTGGACATGTGTCGTGATCCCATCAGTTGGTC17870.11517499896877448No Hit
GTCGTGACTGGGAAAACCCTGGTCGAAAGTCGTGATCAATAGGAGTGACC17700.11407932186610568No Hit
GTCGTGACTGGGAAAACCCGGGCAGATAGTCGTGATCTCGACCCGTGACC17350.11182351606649342No Hit
GTCGTGACTGGGAAAACCCTGGTTACTCGTCGTGATTCGTGTGCCTGACC16950.10924545229550797No Hit
GTCGTGACTGGGAAAACCCTGGATTGTCGTCGTGATATACGGAACTGACC16590.10692519490162108No Hit
GTGGTGACTGGGAAAACCCTGGGATCACGTCGTGATATGAACTCCTGACC16540.1066029369302479No Hit
GTCGTGACTGGGAAAACCCTGGCCAATAGTCGTGATTTTATAACATGACC16520.10647403374169863No Hit
GTGGTGACTGGGAAAACCCTGGATAGCTGTCGTGATGCGAGTACCTGACC16340.10531390504475517No Hit
GTCGTGACTGGGAAAACCCTGGGCAATCGTCGTGATGCCGAAGGTTGACC16020.10325145402796684No Hit
GTCGTGACTGGGAAAACCCTGGATTCGTGTCGTGATTTAGTGTAGTGACC15890.10241358330239655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)