FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567401
Sequences flagged as poor quality0
Sequence length248
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCCAAGTGTCGTGATTAGCAAGTTTTAGG11870.20919949030756027No Hit
GTCGTGACTGGGAAAACCCTGGTGGCACGTCGTGATGCGATAACTTTAGG8610.15174453340759006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTAGG7630.13447279789778305No Hit
GTCGTGACTGGGAAAACCCTGGACTTTAGTCGTGATGACGTTAGATTAGG6790.11966845317509134No Hit
GTCGTGACTGGGAAAACCCTGGGCTGAGGTCGTGTTACAAGTGACTTAGG5780.10186799106804535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC555750.0241.061162
ACTGGGA556450.0240.762637
TGGGAAA561900.0238.405889
GTGACTG563550.0237.834144
CGTGACT564700.0237.198683
TGACTGG566150.0236.914435
GACTGGG573600.0233.795626
CTGGGAA575950.0232.422048
TGCGAAA800.0181.48529
ACGTGAC1750.0179.899442
CGAGACT2050.0177.168013
GACGTGA2050.0147.679081
TCGAGAC2150.0135.165942
CTGCGAA1100.0131.989238
GTCGTGA1080650.0123.948751
ACGAGAC602.2791937E-9121.086162
GACTGGA2000.0121.00086
GTCGAGA2450.0118.625471
ACCTGAC459.375781E-6107.632142
ACCGGGA2200.0104.491487
Produced by FastQC (version 0.11.5)