FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933806
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGACGGACGTCGTGATACTGTTTTGGCCAA97721.046470037673778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT842250.0241.282293
GTGACTG859150.0241.24414
TGACTGG858200.0241.206625
TCGTGAC861350.0241.141742
GACTGGG868300.0240.177066
ACTGGGA875050.0236.845527
TGGGAAA868900.0233.206739
CTGGGAA891200.0231.281688
ACGGGGA13550.0220.622837
TGACGGG13350.0209.489915
CGTGACA11600.0208.907293
CGTGGCT7550.0208.630583
GGTGACT20750.0208.46433
CGTGACG11500.0200.187683
GACGGGG17300.0189.60046
TCGTGGC7350.0186.362722
GTGACGG15450.0184.199374
GTGGTGA30850.0179.252011
GCGTGAC8000.0178.796882
CGTGACC8600.0174.70483
Produced by FastQC (version 0.11.5)