FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301021
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCAGAT18280.14050503412320015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1272150.0241.266923
GTGACTG1273050.0241.079474
TGACTGG1273050.0240.968295
TCGTGAC1275350.0240.952712
ACTGGGA1278050.0239.63747
GACTGGG1280350.0239.433736
CTGGGAA1326650.0230.785648
TGGGAAA1332200.0229.906179
ACCGGGA2350.0149.315937
TCGTGGC4500.0129.237732
GTCGTGA2471350.0124.294981
ACGTGAC2750.0118.957452
CGTGGCT5050.0117.498243
TCGCAAA654.0017767E-9111.685599
CGAGACT3050.0111.16933
CGGGGAA4250.0108.181749
TGACCGG3850.0106.8548365
GCGTGGC3100.0101.618372
TAGATCG2400.0100.831167
TGCGAAA2400.0100.827289
Produced by FastQC (version 0.11.5)