FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826298
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCTGACGGTCGTGATATTTATGCTACTTG18330.22183280124119872No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGACTTG11530.13953803591440375No Hit
GTCGTGACTGGGAAAACCCTGGCCTCCCGTCGTGATAACGAGGGAACTTG11180.13530227593434815No Hit
GTCGTGACTGGGAAAACCCTGGTAGCAGGTCGTGATGATGTGGATACTTG8390.10153721780761904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC813200.0241.222852
GTGACTG812400.0241.076284
CGTGACT813650.0240.883033
TGACTGG813250.0240.827365
ACTGGGA813350.0240.39647
GACTGGG815700.0239.926016
CTGGGAA859750.0227.379878
TGGGAAA889100.0219.99639
GTCGTGA1583950.0123.820731
ACTAGGA1100.0110.00147
GACTAGG1150.0105.22516
ACTCGGA955.456968E-12101.8960347
ACCGGGA1850.098.109357
ACGTGAC2450.093.968732
CGGGGAA2650.091.3274469
TCGAGAC4050.089.75572
TCGTGGC2300.089.560582
GACTGCG1650.088.006456
GACGTGA2350.087.6603551
TGACGGG1800.080.672585
Produced by FastQC (version 0.11.5)