FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964733
Sequences flagged as poor quality0
Sequence length248
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGAACCGCGTCGTGATAAGAAGTACTGACC52080.265074185652707No Hit
GTCGTGACTGGGAAAACCCTGGATCCGCGTCGTGATACCAGCGATTGACC46010.23417940249387575No Hit
GTCGTGACTGGGAAAACCCTGGCACAGTGTCGTGATTCCTGGCCCTGGAC45560.23188901494503325No Hit
GTCGTGACTGGGAAAACCCTGGCTATGAGTCGTGATAAGACCCAATGACC41050.2089342419555227No Hit
GTCGTGACTGGGAAAACCCTGGTTTTTCGTCGTGATGCAATAAGCTGGCG31860.16215943845805003No Hit
GTCGTGACTGGGAAAACCCTGGCAAGCAGTCGTGATGATACTGTGTGACC31070.15813853587230428No Hit
GTCGTGACTGGGAAAACCCTGGCAAGACGTCGTGATCCAACGACATGACC30100.15320147826702152No Hit
GTCGTGACTGGGAAAACCCTGGTCTTTCGTCGTGATCGTGGGGGATGACC29640.15086019321709362No Hit
GTCGTGACTGGGAAAACCCTGGGCCTGTGTCGTGATATGATCACGTGACC29240.14882429317367804No Hit
GTCGTGACTGGGAAAACCCTGGACGGTTGTCGTGATGCCGCCGCTTGACC27260.13874658795877098No Hit
GTCGTGACTGGGAAAACCCTGGTACACAGTCGTGATATGTAGTGTTGACC26720.13599812290015997No Hit
GTCGTGACTGGGAAAACCCTGGAGCGCCGTCGTGATCGGGTGTTTTGACC25720.13090837279162104No Hit
GTCGTGACTGGGAAAACCCTGGTCTCCCGTCGTGATAAGTAGAAGTGACC25010.1272946502145584No Hit
GTCGTGACTGGGAAAACCCTGGTGCCTTGTCGTGATTCTGAGGAATGACC24300.12368092763749577No Hit
GTCGTGACTGGGAAAACCCTGGGTTCCAGTCGTGATCAACAGTGATGACC24280.123579132635325No Hit
GTCGTGACTGGGAAAACCCTGGAGGTCAGTCGTGATGTAAGCATCTGACC24110.12271387511687337No Hit
GTCGTGACTGGGAAAACCCTGGCCGGAGGTCGTGATTTCATTTTGTGACC23100.11757322750724906No Hit
GTCGTGACTGGGAAAACCCTGGTCTTATGTCGTGATACGGGGTACTGACC22350.11375591492584489No Hit
GTCGTGACTGGGAAAACCCTGGAGACCAGTCGTGATAGACATAGCTGACC22130.11263616990196633No Hit
GTCGTGACTGGGAAAACCCTGGGGATGAGTCGTGATTGAAGCGGATGACC21990.11192360488677087No Hit
GTCGTGACTGGGAAAACCCTGGGATATGGTCGTGATCTTAATTCCTGACC21920.11156732237917315No Hit
GTCGTGACTGGGAAAACCCTGGTTATATGTCGTGATATGAAGGAGTGACC21870.1113128348737462No Hit
GTCGTGACTGGGAAAACCCTGGATTCGGGTCGTGATCGGTAGCTTTGACC21800.11095655236614847No Hit
GTCGTGACTGGGAAAACCCTGGAAAAGCGTCGTGATTGGAGGGTATGACC21760.11075296236180691No Hit
GTCGTGACTGGGAAAACCCTGGTTAGCGGTCGTGATGCGCAGGTTTGACC21690.11039667985420919No Hit
GTCGTGACTGGGAAAACCCTGGTTCCTTGTCGTGATCTGTGGGGGTGACC21610.10998949984552608No Hit
GTCGTGACTGGGAAAACCCTGGGGGACTGTCGTGATTGCCGCGGGTGACC21200.10790270230102512No Hit
GTCGTGACTGGGAAAACCCTGGCTAGCCGTCGTGATTCTTGTTCCTGACC21150.10764821479559818No Hit
GTCGTGACTGGGAAAACCCTGGTTACACGTCGTGATCACACCAACTGACC20820.10596859725978033No Hit
GTCGTGACTGGGAAAACCCTGGAAGCAGGTCGTGATATTCATTATTGACC20730.10551051975001183No Hit
GTCGTGACTGGGAAAACCCTGGCACGCTGTCGTGATCAGAGGTCATGACC20580.104747057233731No Hit
GTCGTGACTGGGAAAACCCTGGGTGACCGTCGTGATGCCCAGTTATGACC20540.10454346722938944No Hit
GTCGTGACTGGGAAAACCCTGGTATCTGGTCGTGATGTAGGCAAATGACC20430.10398359471745017No Hit
GTCGTGACTGGGAAAACCCTGGAGGCCCGTCGTGATTGTTAGGTATGACC20300.10332192720334009No Hit
GTCGTGACTGGGAAAACCCTGGTGGAACGTCGTGATGTCAGGTACTGACC20280.10322013220116931No Hit
GTCGTGACTGGGAAAACCCTGGCTCATTGTCGTGATCGCGGAGAGTGACC19930.1014387196631807No Hit
GTCGTGACTGGGAAAACCCTGGTAGATGGTCGTGATGTCCGTACCTGACC19870.10113333465666836No Hit
GTCGTGACTGGGAAAACCCTGGTAAGTAGTCGTGATTGTTCTTTATGACC19750.10052256464364369No Hit
GTCGTGACTGGGAAAACCCTGGTTTTGCGTCGTGATGCATAGTTCTGACC19720.10036987214038752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA1833550.0240.809117
GTGACTG1830950.0240.075234
TCGTGAC1814250.0239.796222
TGACTGG1833150.0239.6935
TGGGAAA1808500.0238.826059
GACTGGG1865400.0237.740486
CTGGGAA1899350.0231.671948
CGTGACT1850000.0231.530383
ACGGGGA37950.0214.340417
GACGGGG43100.0202.791936
CGTGACA25650.0201.705873
CGTGACG24500.0190.40253
GGTGACT61450.0188.303993
TCGAGAC4100.0186.318452
GTGACGG40600.0180.48054
CGGGGAA43550.0180.089838
TGACGGG41300.0177.66955
TGACAGG33700.0160.608285
GTGGTGA91150.0159.420181
GTGACAG35300.0153.710754
Produced by FastQC (version 0.11.5)