FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853343
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCTGGATGTCGTGATAATCTCGGGTGACC26140.3063246549160185No Hit
GTCGTGACTGGGAAAACCCTGGCCCCGCGTCGTGATCCGATATGCTGACC22180.25991893060586424No Hit
GTCGTGACTGGGAAAACCCTGGATTTGTGTCGTGATAGCAAGGGGTGACC14800.17343553530057668No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGACC11820.13851405589546056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA837350.0240.820487
GTGACTG841750.0240.191734
TCGTGAC841750.0240.174642
TGACTGG842800.0239.995385
TGGGAAA842400.0239.448629
GACTGGG852100.0236.992616
CGTGACT863650.0234.002373
CTGGGAA873600.0230.772258
TCGAGAC2300.0136.913862
CGAGACT2400.0136.199693
GACTGGA4500.0131.750616
GTCGTGA1633550.0123.706141
CGGGGAA2050.0112.1421369
GTCGCGA903.6379788E-12107.709311
GACTAGG852.2555469E-1099.643326
TCGCGAC1007.2759576E-1296.892882
GCGTGGC1150.094.7865142
GACTGGC1550.093.67396
ACTGGAA6650.089.154547
CGCGACT1203.8198777E-1180.710933
Produced by FastQC (version 0.11.5)