FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629681
Sequences flagged as poor quality0
Sequence length248
%GC63

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGACCCTGCCGTCGTGATGCCGCGAGATCAC21100.12947319137917176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1526700.0242.04342
CGTGACT1530250.0241.414173
ACTGGGA1513350.0241.341347
TGGGAAA1517400.0240.068229
CGTGGCT59150.0239.433413
GTGACTG1548950.0238.39614
TGACTGG1550500.0238.037815
CTGGGAA1558450.0237.976998
GACTGGG1550800.0237.478586
TCGTGGC47000.0234.47582
CGTTACT950.0216.795533
TCGTTAC1600.0212.163182
GCGTGGC16650.0208.249422
GTCGTGG53850.0205.663621
CGAGACT1200.0201.91743
GTGACTT6850.0199.780354
TGGCTGG77750.0183.899725
CGTGACA3850.0179.365593
GCTGGGG42300.0176.556217
GGCTGGG92300.0171.94096
Produced by FastQC (version 0.11.5)