FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863600
Sequences flagged as poor quality0
Sequence length248
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA2719850.0241.185827
TGACTGG2742800.0240.781285
GTGACTG2744000.0240.659644
TCGTGAC2740700.0240.560642
TGGGAAA2647400.0240.311559
GACTGGG2757050.0239.305136
CTGGGAA2812300.0234.320748
CGTGACT2814200.0233.795583
CGTGGCT46650.0220.718493
GCGTGGC11700.0202.998832
TGGGAAC87450.0171.211239
GTGACTT14750.0163.370294
CGTGACA9050.0160.621663
TCGTGGC56000.0155.583742
CTGCGAA2500.0154.928858
ACTGGGG27250.0147.027637
GGCGTGG17650.0140.808851
GTCGTGG63950.0138.768661
AATGGGA5700.0135.907267
GACTGGA13700.0132.535136
Produced by FastQC (version 0.11.5)