FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908873
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGATCA11380.12521001284007777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT889500.0241.518393
TCGTGAC891750.0241.123292
GTGACTG891500.0240.869144
TGACTGG892450.0240.724445
GACTGGG895200.0239.579716
ACTGGGA895050.0239.363637
CTGGGAA910750.0235.197518
TGGGAAA943850.0226.936529
CGAGACT2400.0131.213363
ACGTGAC1800.0127.919372
GTCGTGA1741100.0123.490181
TCGTGGC3850.0107.022082
CGGGGAA3000.0100.838959
GTGACTA1250.096.874694
GCGTGGC2150.095.82212
CGTGGCT5400.094.204463
CGTGACC3600.087.475573
ACCGGGA3100.085.8757557
GACGTGA2750.083.742881
GTCGTCA1203.8198777E-1180.8045351
Produced by FastQC (version 0.11.5)