FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513943
Sequences flagged as poor quality0
Sequence length248
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTTTATCGTCGTGATGCCAACGGGCAGTG164893.2083324415353456No Hit
GTCGTGACTGGGAAAACCCTGGCTCATTGTCGTGATCGGTCCCCCCAGTG163503.181286640736424No Hit
GTCGTGACTGGGAAAACCCTGGTAATTTGTCGTGATGAAAGGTTGCAGTG146572.851872678487692No Hit
GTCGTGACTGGGAAAACCCTGGAACTTTGTCGTGATTGCTATCCGCAGTG136842.6625520728952434No Hit
GTCGTGACTGGGAAAACCCTGGGTATTGGTCGTGATAACTGCCCGCAGTG129722.524015309090697No Hit
GTCGTGACTGGGAAAACCCTGGGATGTCGTCGTGATGGCCTGCCGCAGTG125462.4411267397357292No Hit
GTCGTGACTGGGAAAACCCTGGGCACTCGTCGTGATAGTGTCCCGCAGTG121502.364075393574774No Hit
GTCGTGACTGGGAAAACCCTGGCTCCGTGTCGTGATTCTGGCCCGCAGTG120432.3432559641827986No Hit
GTCGTGACTGGGAAAACCCTGGTCCTGCGTCGTGATACGCTAGGGCAGTG114882.2352673350935803No Hit
GTCGTGACTGGGAAAACCCTGGAGTCCAGTCGTGATTCAGACCGTCAGTG114772.233127019922443No Hit
GTCGTGACTGGGAAAACCCTGGATTGCTGTCGTGATCGGGAGGTGCAGTG105592.054507990185682No Hit
GTCGTGACTGGGAAAACCCTGGGAGTTAGTCGTGATGGATGCCGTCAGTG97931.9054642246319147No Hit
GTCGTGACTGGGAAAACCCTGGCCGACTGTCGTGATGCTGAATAGCAGTG92321.796308150903894No Hit
GTCGTGACTGGGAAAACCCTGGACTGATGTCGTGATGGAAATTTGCAGTG91081.7721809617019788No Hit
GTCGTGACTGGGAAAACCCTGGCTTTTCGTCGTGATTGCATTCCGCAGTG87401.7005776905221006No Hit
GTCGTGACTGGGAAAACCCTGGTTTGCTGTCGTGATACACATGGGCAGTG84421.642594606794917No Hit
GTCGTGACTGGGAAAACCCTGGGTCCGTGTCGTGATTCAACCAAACAGTG84181.6379248282397074No Hit
GTCGTGACTGGGAAAACCCTGGGATTCCGTCGTGATGAAGATCCGCAGTG82461.604458081927373No Hit
GTCGTGACTGGGAAAACCCTGGTGGCAGGTCGTGATTTATCCCCCCAGTG80881.573715373105578No Hit
GTCGTGACTGGGAAAACCCTGGATGCCCGTCGTGATGTCTAACCTCAGTG78281.5231261054241423No Hit
GTCGTGACTGGGAAAACCCTGGGCTAAGGTCGTGATTAACTCTCGCAGTG67031.3042302356487003No Hit
GTCGTGACTGGGAAAACCCTGGCCCCCCGTCGTGATCAAAGGGTGCAGTG66181.2876914365990004No Hit
GTCGTGACTGGGAAAACCCTGGCAGTTCGTCGTGATGTGAACGATCAGTG46120.8973757790260788No Hit
GTCGTGACTGGGAAAACCCTGGCCATCAGTCGTGATGCGAAGTAGCAGTG45920.8934842968967375No Hit
GTCGTGACTGGGAAAACCCTGGACTAGTGTCGTGATTAGTGAAACCAGTG31840.6195239549911178No Hit
GTCGTGACTGGGAAAACCCTGGCCCGAGTCCTGATATGTAGTCCGCAGTG31550.6138813059035729No Hit
GTCGTGACTGGGAAAACCCTGGATTGGTGTCGTGATATCCAGAACCAGTG28000.5448074981077669No Hit
GTCGTGACTGGGAAAACCCTGGCGGCGTGTCGTGATCTGGATGGGCAGTG26410.5138702151795044No Hit
GTCGTGACTGGGAAAACCCTGGGACATGGTCGTGATTCTTAGTATCAGTG26030.5064763991337561No Hit
GTCGTGACTGGGAAAACCCTGGCGAGGTGTCGTGATGTATTAGAACAGTG25960.5051143803884867No Hit
GTCGTGACTGGGAAAACCCTGGCCCTCCGTCGTGATCGCGAGGTCCAGTG24780.4821546358253736No Hit
GTCGTGACTGGGAAAACCCTGGGGCTCAGTCGTGATAGATGGTTTCAGTG24370.4741770974602242No Hit
GTCGTGACTGGGAAAACCCTGGACCAATGTCGTGATCCAATAGAACAGTG23370.45471968681351826No Hit
GTCGTGACTGGGAAAACCCGGGTTTATCGTCGTGATGCCAACGGGCAGTG23210.45160650111004524No Hit
GTCGTGACTGGGAAAACCCTGGAGCACTGTCGTGATGATCCCCCGCAGTG21940.4268955895887287No Hit
GTCGTGACTGGGAAAACCCTGGATGCCCATCGTGATGTCTAACCTCAGTG20150.39206682453112507No Hit
GTCGTGACTGGGAAAACCCGGGTAATTTGTCGTGATGAAAGGTTGCAGTG19640.382143545101305No Hit
GTCGTGACTGGGGAAATCCCTGGGGCTCAGTCGTGATAGATGGTTCAGTG19300.375528025481425No Hit
GTCGTGACTGGGAAAACCCTGGCCCGGGGTCGTGATGGTGCAGCGCAGTG18970.369107079968012No Hit
GTCGTGACTGGGAAAACCCGGGGTATTGGTCGTGATAACTGCCCGCAGTG18650.36288070856106613No Hit
GTCGTGACTGGGAAAACCCTGGCAGCGAGTCGTGATTAGTGAAACCAGTG17550.34147755684968956No Hit
GTCGTGACTGGGAAAACCCTGGCTGCACGTCGTGATTTACGCGTGCAGTG16920.3292193881422648No Hit
GTCGTGACTGGGAAAACCCGGGGAGTTAGTCGTGATGGATGCCGTCAGTG16700.3249387577999895No Hit
GTCGTGACTGGGAAAACCCTGGCCCGAGTCGTGATATGTAGTCCGCAGTG14950.29088828916825404No Hit
GTCGTGACTGGGAAAACCCTGGCCTCTCGTCGTGATGCACAGAACCAGTG14330.27882469456729636No Hit
GTCGTGACTGGGAAAACCCGGGCTTTTCGTCGTGATTGCATTCCGCAGTG14110.27454406422502103No Hit
GTCGTGACTGGGAAAACCCTGGATCCTCGTCGTGATCTGACGTCGCAGTG13700.2665665258598716No Hit
GTCGTGACTGGAAAAACCCTGGAACTTTGTCGTGATTGCTATCCGCAGTG13120.2552812276847822No Hit
GTCGTGACTGGGAAAACCCGGGTTTGCTGTCGTGATACACATGGGCAGTG12960.2521680419813092No Hit
GTCGTGACTGGGAAAACCCGGGATTGCTGTCGTGATCGGGAGGTGCAGTG12510.24341220719029155No Hit
GTCGTGACTGGGAAAACCCTGGATGGTTGTCGTGATAGTGTCCCGCAGTG11450.22278735190478322No Hit
GTCGTGACTGGGAAAACCCGGGAGTCCAGTCGTGATTCAGACCGTCAGTG11130.2165609804978373No Hit
GTCGTGACTGGGGAAAACCCTGGGGCTCAGTCGTGATAGATGGTTCAGTG10890.2118912019426279No Hit
GTCGTGACTGGGAAAACCCTGGTTGACTGTCGTGATATTATGTTGCAGTG10190.19827101448993373No Hit
GTCGTGACTGGGAAAACCCGGGCTCATTGTCGTGATCGGTCCCCCCAGTG10010.19476868057352664No Hit
GTCGTGACTGGGAAAACCCGGGAACTTTGTCGTGATTGCTATCCGCAGTG9620.18718029042131132No Hit
GTCGTGACTGGGAAAACCCTGGTACGCAGTCGTGATCTTAATGTCACAGT9600.1867911422083772No Hit
GTCGTGACTGGGAAAACCCGGGGCTAAGGTCGTGATTAACTCTCGCAGTG9460.18406710471783838No Hit
GTCGTGACTGGGAAAACCCTGGCCCATGGTCGTGATGTGCAGGTCCAGTG8920.17356010296861715No Hit
GTCGTGACTGGGAAAACCCTGGCCCTGAGTCGTGATGCTGCGGTCCAGTG7770.1511840807249053No Hit
GTCGTGACTGGGAAAACCCTGGCAACTTGTCGTGATTATAGGTAGCAGTG7700.14982206197963588No Hit
GTCGTGACTGGGAAAACCCGGGGATGTCGTCGTGATGGCCTGCCGCAGTG7340.1428173941468217No Hit
GTCGTGACTGGGAAAACCCGGGGATTCCGTCGTGATGAAGATCCGCAGTG7010.13639644863340877No Hit
GTGGTGACTGGGAAAACCCTGGTAATTTGTCGTGATGAAAGGTTGCAGTG6510.12666774331005579No Hit
GTCGTGACTGGGAAAACCCTGGCCGCTGGTCGTGATGTTAGCGTCCAGTG6510.12666774331005579No Hit
GTGGTGACTGGGAAAACCCTGGAACTTTGTCGTGATTGCTATCCGCAGTG6450.12550029867125342No Hit
GTCGTGACTGGGAAAACCCGGGTGGCAGGTCGTGATTTATCCCCCCAGTG6150.11966307547724164No Hit
GTGGTGACTGGGAAAACCCTGGTTTATCGTCGTGATGCCAACGGGCAGTG5920.11518787102849928No Hit
GTGGTGACTGGGAAAACCCGGGTAATTTGTCGTGATGAAAGGTTGCAGTG5800.11285298175089456No Hit
GTCGTGACTGGGAAAACCCTGGCCCAAGGTCGTGATCACCCCGTCCAGTG5200.10117853536287097No Hit
GTGGTGACTGGGAAAACCCGGGTTTATCGTCGTGATGCCAACGGGCAGTG5180.10078938714993688No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAC100.001520348242.587362
AGGTGAC100.001520348242.587362
CGTGACT445250.0242.441863
TAACTGG100.001525683242.303735
TCGGAAA400.0242.185739
ACTCGGA350.0242.162147
TCGTGAC454500.0242.107012
ACTGGGA471050.0241.802287
GACTGGG477150.0241.725236
TGGGAAA453500.0241.624979
GTGACTG472750.0241.57994
CTGGGAA470050.0241.440878
TGGTGAC30250.0238.577642
CGGGGAA11500.0235.844868
TGACTGG486150.0234.653065
GGTGACT26850.0234.341643
GTGGTGA32550.0232.63961
GGACTGG8700.0229.770755
GGGACTG8750.0227.139574
TGACTGA750.0226.150135
Produced by FastQC (version 0.11.5)