FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414328
Sequences flagged as poor quality0
Sequence length248
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGACTCGCGTCGTGATCGAATCAGCTAGCT93302.2518391226274836No Hit
GTCGTGACTGGGAAAACCCTGGAGAGATGTCGTGATTGACCGTTCTAGCT85612.06623737715047No Hit
GTCGTGACTGGGAAAACCCTGGCACACCGTCGTGATACTGTGTTCTAGCT84682.0437913923268525No Hit
GTCGTGACTGGGAAAACCCTGGCGACCAGTCGTGATGTTGTGTTCTAGCT84232.0329304319283272No Hit
GTCGTGACTGGGAAAACCCTGGTTGTGGGTCGTGATCATAGAGGATAGCT81351.9634202853777685No Hit
GTCGTGACTGGGAAAACCCTGGGCGTTAGTCGTGATGCAAATAACTAGCT80831.9508698422505841No Hit
GTCGTGACTGGGAAAACCCTGGTCTCTTGTCGTGATCCGGACGACTAGCT80501.9429051379583326No Hit
GTCGTGACTGGGAAAACCCTGGTAGCTCGTCGTGATGCTTCGTTCTAGCT71911.7355814716842692No Hit
GTCGTGACTGGGAAAACCCTGGCCGACAGTCGTGATGCTTACGTTTAGCT68061.6426599216080016No Hit
GTCGTGACTGGGAAAACCCTGGACTACTGTCGTGATCCAGTGTTCTAGCT66141.5963198239076288No Hit
GTCGTGACTGGGAAAACCCTGGCTCTCGGTCGTGATGGACTGTTCTAGCT64121.5475661794520283No Hit
GTCGTGACTGGGAAAACCCTGGCTCCCGGTCGTGATGGAAAGTTCTAGCT62991.5202931011179548No Hit
GTCGTGACTGGGAAAACCCTGGGAGAGTGTCGTGATGCTTAGAGCTAGCT62471.5077426579907707No Hit
GTCGTGACTGGGAAAACCCTGGTGTCAGGTCGTGATAAATGGTGATAGCT61601.486744801220289No Hit
GTCGTGACTGGGAAAACCCTGGCGAGGCGTCGTGATAGAGCTCAATAGCT55611.3421733505821476No Hit
GTCGTGACTGGGAAAACCCTGGGGAGAAGTCGTGATAGAAGGAAATAGCT54931.3257612326465988No Hit
GTCGTGACTGGGAAAACCCTGGATAAACGTCGTGATCTATCGTGCTAGCT53951.302108474445367No Hit
GTCGTGACTGGGAAAACCCTGGCCTTGGGTCGTGATGAGCGGTTCTAGCT53851.299694927690139No Hit
GTCGTGACTGGGAAAACCCTGGCACCTGGTCGTGATGAGCGACTATAGCT52731.2726632040315884No Hit
GTCGTGACTGGGAAAACCCTGGGGTATGGTCGTGATCAACGGTGATAGCT51001.2309088451661485No Hit
GTCGTGACTGGGAAAACCCTGGCCCGGTGTCGTGATAGGGAACCCTAGCT50111.2094282790446216No Hit
GTCGTGACTGGGAAAACCCTGGGCACGTGTCGTGATGAGTAGTATTAGCT48971.1819138460350254No Hit
GTCGTGACTGGGAAAACCCTGGGTCTCTGTCGTGATAAAAATTGCTAGCT47631.1495723195149736No Hit
GTCGTGACTGGGAAAACCCTGGTGCCAAGTCGTGATTATAGCCCCTAGCT45561.0996119016817594No Hit
GTCGTGACTGGGAAAACCCTGGCCCTCGGTCGTGATATGACGTTCTAGCT43491.0496514838485451No Hit
GTCGTGACTGGGAAAACCCTGGACCTCGTCCTGATAGAAGCTTCCTAGCT43181.0421694889073392No Hit
GTCGTGACTGGGAAAACCCTGGCTGTTACGAGCAAGGCGTCGAGATAGCT41941.0122415091425152No Hit
GTCGTGACTGGGAAAACCCTGGCCGTTAGTCGTGATCCCAACTTCTAGCT40810.9849684308084417No Hit
GTCGTGACTGGGAAAACCCTGGGACCCAGTCGTGATCCGGACGACTAGCT40700.9823135293776911No Hit
GTCGTGACTGGGAAAACCCTGGGAGCTCGTCGTGATGTTACGTTCTAGCT38830.9371802050549324No Hit
GTCGTGACTGGGAAAACCCTGGTAGCACGTCGTGATCGAATCAGCTAGCT38140.920526732443861No Hit
GTCGTGACTGGGAAAACCCTGGTTGAAAGTCGTGATCGCATGTCATAGCT38070.9188372497152014No Hit
GTCGTGACTGGGAAAACCCTGGAATATTGTCGTGATATAATTCCCTAGCT36240.8746693440945338No Hit
GTCGTGACTGGGAAAACCCTGGAACCGCGTCGTGATCCTTACCAGTAGCT35370.8536714873240524No Hit
GTCGTGACTGGGAAAACCCTGGACGCCCGTCGTGATTCACCGCCCTAGCT32110.774989863103628No Hit
GTCGTGACTGGGAAAACCCTGGTCCTCGGTCGTGATGCCCAGGTCTAGCT30560.737579888397598No Hit
GTCGTGACTGGGAAAACCCTGGGACCACGTCGTGATACGTATGACTAGCT29710.7170647409781622No Hit
GTCGTGACTGGGAAAACCCTGGCCTCGGGTCTGATAGTGTGTTCCTAGCT29700.7168233863026394No Hit
GTCGTGACTGGGAAAACCCTGGCCCTATGTCGTGATACTTAACAATAGCT28590.6900330173196115No Hit
GTCGTGACTGGGAAAACCCTGGCGACCGTCCTGATGTTGTGTTCCTAGCT27820.6714487073043579No Hit
GTCGTGACTGGGAAAACCCTGGAATTATGTCGTGATCGCATCTAGTAGCT27050.6528643972891043No Hit
GTCGTGACTGGGAAAACCCTGGTTGCATGTCGTGTTCCCACCCGATAGCT26350.6359695700025101No Hit
GTCGTGACTGGGAAAACCCTGGCCTTCCGTCGTGATGAAAACTCATAGCT25630.6185920333648703No Hit
GTCGTGACTGGGAAAACCCTGGACGCCTGTCGTGATAATGTGTTCTAGCT24830.5992836593230484No Hit
GTCGTGACTGGGAAAACCCTGGTATGCGGTCGTGATAATTCGTCCTAGCT24250.5852850881427275No Hit
GTCGTGACTGGGAAAACCCTGGCCCCTTTCCTGATCAATATCTAGTAGCT24100.5816647680098859No Hit
GTCGTGACTGGGAAAACCCTGGTTATTAGTCGTGATTATTACCAATAGCT23260.5613909752659728No Hit
GTCGTGACTGGGAAAACCCTGGGCCCCGGTCGTGATGTAGACCAATAGCT20330.4906740553378No Hit
GTCGTGACTGGGAAAACCCTGGTTCAGGGTCGTGATTTTAATCCCTAGCT20000.48270935104554846No Hit
GTCGTGACTGGGAAAACCCTGGATGCTAGTCGTGATTCGGTTACCTAGCT19840.4788476762371841No Hit
GTCGTGACTGGGAAAACCCTGGGGGGGGGTCGTGATTCGCTGAATTAGCT18910.4564016914135661No Hit
GTCGTGACTGGGAAAACCCTGGCTAGGAGTCGTGATAAGTTGGAATAGCT18660.45036782452549673No Hit
GTCGTGACTGGGAAAACCCTGGTAAAGCGTCGTGATTAGCCCCCATAGCT18450.44529937633951844No Hit
GTCGTGACTGGGAAAACCCTGGTTGCATGTCGTGTTCCCACCAGATAGCT17230.41585410592574No Hit
GTCGTGACTGGGAAAACCCTGGACCCTCGTCGTGTCAACGGGGGTTAGCT17120.4131992044949895No Hit
GTCGTGACTGGGAAAACCCTGGCTCGGTGTCGTGATCTGAATGCATAGCT17000.41030294838871617No Hit
GTCGTGACTGGGAAAACCCTGGACCTCGTCGTGATAGAAGCTTCCTAGCT13990.3376551910563611No Hit
GTCGTGACTGGGAAAACCCTGGCGACCGTCGTGATGTTGTGTTCCTAGCT13780.33258674287038287No Hit
GTCGTGACTGGGAAAACCCTGGGGCAATGTCGTGATCACAATCCATAGCT12390.29903844297271726No Hit
GTCGTGACTGGGAAAACCCTGGCCCCCGGTCGTGATCCACGACCCTAGCT12060.2910737386804657No Hit
GTCGTGACTGGGAAAACCCTGGCCCCTTTCGTGATCAATATCTAGTAGCT11760.2838330984147825No Hit
GTCGTGACTGGGAAAACCCTGGAGGGTGGTCGTGATATGGACTCCTAGCT11580.2794887142553726No Hit
GTCGTGACTGGGAAAACCCTGGGGTCCTGTCGTGATGATAGACTTTAGCT11440.2761097487980537No Hit
GTCGTGACTGGGAAAACCCTGGGGTGTCGTCGTGATCTAATATCGTAGCT10150.2449749956556158No Hit
GTCGTGACTGGGAAAACCCTGGAGAGTTGTCGTGATTTACCGTCATAGCT9610.23194184317738603No Hit
GTCGTGACTGGGAAAACCCTGGCGAGCGGTCGTGATTATTAGTAGTAGCT8750.21118534108242748No Hit
GTCGTGACTGGGAAAACCCTGGTTACGTGTCGTGATTACCTGTTCTAGCT8340.2012897993859937No Hit
GTCGTGACTGGGAAAACCCTGGATGGACGTCGTGATAGAGTGTTCTAGCT8080.19501457782240159No Hit
GTCGTGACTGGGAAAACCCTGGGTGAGGTCCTGATAAACGGTTCCTAGCT7350.17739568650923906No Hit
GTCGTGACTGGGAAAACCCTGGGCTAAGGTCGTGATTAACTCTCGTAGCT6880.16605201675966866No Hit
GTCGTGACTGGGAAAACCCTGGTATGCGGTCGTGATAATTAGTCCTAGCT6810.16436253403100926No Hit
GTCGTGACTGGGAAAACCCTGGTAAGTAGTCGTGATTTGTATCAATAGCT6780.16363847000444093No Hit
GTCGTGACTGGGAAAACCCTGGCGACCGTCCTGATTTTGTGTTCCTAGCT6750.1629144059778726No Hit
GTCGTGACTGGGAAAACCCTGGTACCAGGTCGTGATTAGCGGTTCTAGCT6460.15591512038771216No Hit
GTCGTGACTGGGAAAACCCTGGCCTTGAGTCGTGATGAAGTCTCGTAGCT6440.1554324110366666No Hit
GTCGTGACTGGGAAAACCCTGGTGAGCGGTCGTGATACGGTGGATTGGTT6290.15181209090382497No Hit
GTCGTGACTGGGAAAACCCTGGTTTATCGTCGTGATGACTCCGGGTAGCT6150.14843312544650614No Hit
GTCGTGACTGGGAAAACCCTGGATGGCTGTCGTGATACTCTGTTCTAGCT6080.14674364271784673No Hit
GTCGTGACTGGGAAAACCCGGGTCCTCGGTCGTGATGCCCAGGTCTAGCT6030.14553686934023286No Hit
GTCGTGACTGGGAAAACCCTGGATAGGTGTCGTGATCTCGTGTTCTAGCT5950.14360603193605068No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGCGTCGTGATAAGCTTTCATAGCT5640.13612403699484465No Hit
GTCGTGACTGGGAAAACCCTGGTTCAGGGTCGTGATTTTAATACCTAGCT5600.13515861829275355No Hit
GTCGTGACTGGGAAAACCCTGGTCCACTGTCGTGATTGGCTGTTCTAGCT5460.13177965283543472No Hit
GTCGTGACTGGGAAAACCCTGGTTTAGCGTCGTGATAGGATGTTCTAGCT5330.12864204205363866No Hit
GTCGTGACTGGGAAAACCCTGGGTAAAAGTCGTGATCACGTTGAATAGCT5290.12767662335154759No Hit
GTCGTGACTGGGAAAACCCGGGGCGTTAGTCGTGATGCAAATAACTAGCT5250.12671120464945645No Hit
GTCGTGACTGGGAAAACCCTGGACCGCGGTCGTGATATTATTAACTAGCT5200.12550443127184258No Hit
GTCGTGACTGGGAAAACCCTGGCCATCAGTCGTGATAAGAATCCCTAGCT5120.12357359386766041No Hit
GTCGTGACTGGGAAAACCCTGGCCTCCAGTCGTGATTTCGAGACCTAGCT4920.11874650035720494No Hit
GTCGTGACTGGGAAAACCCTGGGTGAGGTCCTGATAAACGGTTACTAGCT4800.11585024425093164No Hit
GTCGTGACTGGGAAAACCCTGGTTGCATGTCGTGTTCCCACCAAATAGCT4740.11440211619779497No Hit
GTCGTGACTGGGAAAACCCTGGAGGACCGTCGTGATGTTGTGTTCTAGCT4380.10571334787897511No Hit
GTGGTGACTGGGAAAACCCTGGTCTCTTGTCGTGATCCGGACGACTAGCT4320.10426521982583846No Hit
GTCGTGACTGGGAAAACCCTGGCTGTTACGAGCAATGCGTCGAGATAGCT4200.10136896371956518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC450.0242.407442
CGTTACG151.5875814E-5242.290253
AGTGACT151.5875814E-5242.290253
TCGTGAC374200.0242.27792
CGTGACT369350.0242.224673
GTTACGG201.6525337E-7242.202454
GTGACTT301.8189894E-11242.202454
TGACTGC201.653516E-7242.17325
TGACTGG377900.0242.045035
GTGACTG377900.0241.978134
GACTGGG382600.0241.576846
TGGGAAA382950.0235.732289
GGTGACT8650.0233.887133
TGGTGAC10300.0232.993562
TCGTGGC2050.0224.670322
TGAGTGG1700.0220.804985
GTTACCG500.0217.982214
TTACCGG500.0217.955895
CGTGAGT1450.0217.225773
TGACTTG355.2750693E-11207.577045
Produced by FastQC (version 0.11.5)