FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242296
Sequences flagged as poor quality0
Sequence length248
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGAACCTAGTCGTGATGTTGACGGACGATG4270.17623072605408263No Hit
GTCGTGACTGGGAAAACCCTGGTGAGCCGTCGTGATCTTTGCTATCGATG4040.1667382045101859No Hit
GTCGTGACTGGGAAAACCCTGGTATTCTGTCGTGATCTGAACTGCCGATG4010.16550004952619934No Hit
GTCGTGACTGGGAAAACCCTGGACAACTGTCGTGATATTAAATACCGATG3970.16384917621421732No Hit
GTCGTGACTGGGAAAACCCTGGCACAAGGTCGTGATAAAAGTCGGCGATG3630.14981675306237No Hit
GTCGTGACTGGGAAAACCCTGGAAACCCGTCGTGATTGAATCGGCCGATG3420.14114966817446428No Hit
GTCGTGACTGGGAAAACCCTGGCACTCAGTCGTGATTTGGCGGATCGATG3410.14073694984646878No Hit
GTCGTGACTGGGAAAACCCTGGCACAGTGTCGTGATACTGGCATTCGATG3400.14032423151847329No Hit
GTCGTGACTGGGAAAACCCTGGGAAGCCGTCGTGATGCTAGTGATCGATG3390.13991151319047776No Hit
GTCGTGACTGGGAAAACCCTGGTAGATTGTCGTGATTAAATACCTCGATG3300.1361970482385182No Hit
GTCGTGACTGGGAAAACCCTGGACAACTGTCGTGATGTCGACATTCGATG3200.1320698649585631No Hit
GTCGTGACTGGGAAAACCCTGGAAGATTGTCGTGATATCACAGTTCGATG3070.12670452669462146No Hit
GTCGTGACTGGGAAAACCCTGGCTTTGCGTCGTGATACAGAGGATCGATG3070.12670452669462146No Hit
GTCGTGACTGGGAAAACCCTGGAGCATGGTCGTGATACTGCCCTCCGATG3070.12670452669462146No Hit
GTCGTGACTGGGAAAACCCTGGAGCCATGTCGTGATTCCCCGTACCGATG2940.12133918843067983No Hit
GTCGTGACTGGGAAAACCCTGGTTGCCCGTCGTGATACCCTGTTGCGATG2890.11927559679070228No Hit
GTCGTGACTGGGAAAACCCTGGAAATAGGTCGTGATAGGAGAGCCCGATG2820.11638656849473372No Hit
GTCGTGACTGGGAAAACCCTGGGAGCCTGTCGTGATAAAAAGTTTCGATG2790.11514841351074719No Hit
GTCGTGACTGGGAAAACCCTGGTTGAAAGTCGTGATAACGACGGGCGATG2760.11391025852676066No Hit
GTCGTGACTGGGAAAACCCTGGCTGGCCGTCGTGATCTTAACACTCGATG2750.11349754019876514No Hit
GTCGTGACTGGGAAAACCCTGGTATATCGTCGTGATCTCAAATGACGATG2700.11143394855878759No Hit
GTCGTGACTGGGAAAACCCTGGATGCGTGTCGTGATGAACTAGTACGATG2670.11019579357480107No Hit
GTCGTGACTGGGAAAACCCTGGGTCATAGTCGTGATGGAAAGTCCCGATG2670.11019579357480107No Hit
GTCGTGACTGGGAAAACCCTGGTTAGGAGTCGTGATCCCTGAAGGCGATG2600.1073067652788325No Hit
GTCGTGACTGGGAAAACCCTGGGTGCCAGTCGTGATCCTAACGCACGATG2590.10689404695083699No Hit
GTCGTGACTGGGAAAACCCTGGGCCCTAGTCGTGATTCTCTAAGCCGATG2590.10689404695083699No Hit
GTCGTGACTGGGAAAACCCTGGCTGGCGGTCGTGATCTTTAGTCGCGATG2560.10565589196685048No Hit
GTCGTGACTGGGAAAACCCTGGACGGTTGTCGTGTAGCAATGGTCCGATG2560.10565589196685048No Hit
GTCGTGACTGGGAAAACCCTGGCGTGTAGTCGTGATCCCAAGTACCGATG2550.10524317363885495No Hit
GTCGTGACTGGGAAAACCCTGGCGCCAGGTCGTGATTAGTATTCCCGATG2540.10483045531085944No Hit
GTCGTGACTGGGAAAACCCTGGAACAATGTCGTGATAAGTGGAGCCGATG2540.10483045531085944No Hit
GTCGTGACTGGGAAAACCCTGGTACCAGGTCGTGATGTACTGTTGCGATG2540.10483045531085944No Hit
GTCGTGACTGGGAAAACCCTGGACATTAGTCGTGATGGTTATTAGCGATG2480.1023541453428864No Hit
GTCGTGACTGGGAAAACCCTGGAAACTCGTCGTGATATAGAGAAACGATG2460.10152870868689536No Hit
GTCGTGACTGGGAAAACCCTGGAACCGTGTCGTGATTAGCAAAGACGATG2450.10111599035889986No Hit
GTCGTGACTGGGAAAACCCTGGTAGATCGTCGTGATCAAAAGGGTCGATG2440.10070327203090434No Hit
GTCGTGACTGGGAAAACCCTGGGCGTTTGTCGTGATGAGATTGCACGATG2430.10029055370290885No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGAC100.0015258511242.268432
CCGTGAC100.0015258511242.268432
TCGTGAC224650.0241.513552
CGTGACT222100.0240.750893
ACTGGGA225300.0240.725337
TGGGAAA220800.0239.431789
GTGACTG232250.0234.191654
CTGGGAA231050.0234.101878
TGACTGG232250.0233.622045
GACTGGG234600.0232.214316
ACGTGAC550.0220.244032
CGTGACG2200.0220.153053
CGTGACA3050.0218.34853
CGTGGCT1700.0199.432773
GACGGGG3650.0189.012276
TGGTGAC6000.0183.720232
GTGACAG3650.0182.455614
TGACAGG3650.0182.417915
GGTGACT5600.0177.301833
ACGGGGA3700.0176.644457
Produced by FastQC (version 0.11.5)