FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770150
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1683350.0241.516343
TGACTGG1687200.0241.310475
TCGTGAC1693350.0240.915662
GACTGGG1696300.0239.85936
GTGACTG1697100.0239.821824
ACTGGGA1690250.0239.099537
CTGGGAA1715750.0236.957158
CGTGGCT36000.0231.792453
TGGGAAA1716050.0230.455969
TCGTGGC29550.0227.954042
GCGTGGC10050.0195.289522
GTCGTGG39900.0171.942171
GTGGCTG50250.0170.085424
TGACTTG9000.0166.759935
AGGGAAA8150.0163.346869
GCGGGGC8750.0161.99732
CGTGACA6650.0154.803183
GGCGTGG13200.0152.43091
ACTGGGG19250.0151.525797
GTGACTT10000.0151.324234
Produced by FastQC (version 0.11.5)