FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546306
Sequences flagged as poor quality0
Sequence length248
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCCGCTTGTCGTGATGTAGAAGATTTAGG28020.18120604847940835No Hit
GTCGTGACTGGGAAAACCCTGGGAAATTGTCGTGATCGCAAGAGCTTAGG23930.15475591506467673No Hit
GTCGTGACTGGGAAAACCCTGGCCGTTAGTCGTGATCAGTAGCCTTTAGG22530.14570207966599108No Hit
GTCGTGACTGGGAAAACCCTGGCCCCTAGTCGTGATATTGAATTTTTAGG22110.14298592904638538No Hit
GTCGTGACTGGGAAAACCCTGGCCCTTCGTCGTGATACCAAGTGATTAGG21990.14220988601221235No Hit
GTCGTGACTGGGAAAACCCTGGAATGCCGTCGTGATTTGCGGTTATTAGG21980.14214521575936456No Hit
GTCGTGACTGGGAAAACCCTGGAGTGTCGTCGTGATGTCGCGACATTAGG21320.13787697907141278No Hit
GTCGTGACTGGGAAAACCCTGGTCAACAGTCGTGATGCAAAGAAGTTAGG21130.13664824426730543No Hit
GTCGTGACTGGGAAAACCCTGGCAGTGCGTCGTGATGGCATTGGGTTAGG20860.13490214744041606No Hit
GTCGTGACTGGGAAAACCCTGGTTAGCGTCGTGATAGGCTGAGGTTTAGG20840.13477280693472055No Hit
GTCGTGACTGGGAAAACCCTGGGCCGCTGTCGTGATGACATTGTGTTAGG20650.13354407213061323No Hit
GTCGTGACTGGGAAAACCCTGGAAACCTGTCGTGATGATTAGTCGTTAGG20510.13263868859074465No Hit
GTCGTGACTGGGAAAACCCTGGATGACGGTCGTGATTTTTAGTTCTTAGG20280.1311512727752463No Hit
GTCGTGACTGGGAAAACCCTGGGTGGTCGTCGTGATGGTCAGTGCTTAGG20060.1297285272125957No Hit
GTCGTGACTGGGAAAACCCTGGGGTTTAGTCGTGATGCCAATGGGTTAGG19970.1291464949369659No Hit
GTCGTGACTGGGAAAACCCTGGTGACGCGTCGTGATGAACTGCAGTTAGG19820.1281764411442496No Hit
GTCGTGACTGGGAAAACCCTGGGTTAGGGTCGTGATCGTGGAGTTTTAGG18910.12229144813510393No Hit
GTCGTGACTGGGAAAACCCTGGCCTGTAGTCGTGATACTGACCCTTTAGG18910.12229144813510393No Hit
GTCGTGACTGGGAAAACCCTGGAATTCGGTCGTGATAACTATACCTTAGG18910.12229144813510393No Hit
GTCGTGACTGGGAAAACCCTGGCTTTCTGTCGTGATTGTCGGGAGTTAGG18780.12145073484808311No Hit
GTCGTGACTGGGAAAACCCTGGGAACCAGTCGTGATGTGGAGGACTTAGG18710.12099804307814882No Hit
GTCGTGACTGGGAAAACCCTGGAGAGATGTCGTGATTCAGTATACTTAGG18690.12086870257245332No Hit
GTCGTGACTGGGAAAACCCTGGACTCCCGTCGTGATACAGGTCCCTTAGG18670.12073936206675781No Hit
GTCGTGACTGGGAAAACCCTGGCTGCCAGTCGTGATCTGAACACTTTAGG18580.12015732979112802No Hit
GTCGTGACTGGGAAAACCCTGGACAACGGTCGTGATGGTCGAGGCTTAGG18560.1200279892854325No Hit
GTCGTGACTGGGAAAACCCTGGTGCGTTGTCGTGATATTATGTATTTAGG18460.11938128675695496No Hit
GTCGTGACTGGGAAAACCCTGGGATCTTGTCGTGATTGAGACCACTTAGG18450.1193166165041072No Hit
GTCGTGACTGGGAAAACCCTGGTTTACGGTCGTGATCTCATCTGGTTAGG18450.1193166165041072No Hit
GTCGTGACTGGGAAAACCCTGGACACTGGGTGTATGATAGGTAACTTAGG18380.11886392473417294No Hit
GTCGTGACTGGGAAAACCCTGGCTGCCAGTCGTGATTACAAGATGTTAGG18250.11802321144715212No Hit
GTCGTGACTGGGAAAACCCTGGTTCAGCGTCGTGATGCCTTATTGTTAGG18150.11737650891867457No Hit
GTCGTGACTGGGAAAACCCTGGCATTTTGTCGTGATCCGTGGGATTTAGG18020.11653579563165375No Hit
GTCGTGACTGGGAAAACCCTGGAGAAGAGTCGTGATACAAAGATGTTAGG18000.11640645512595825No Hit
GTCGTGACTGGGAAAACCCTGGGATTAAGTCGTGATGCTTGGAATTTAGG17950.11608310386171947No Hit
GTCGTGACTGGGAAAACCCTGGGATTGAGTCGTGATCGCGAACCCTTAGG17920.11588909310317622No Hit
GTCGTGACTGGGAAAACCCTGGGCACACGTCGTGATCCCCTAAGTTTAGG17620.11394898551774359No Hit
GTCGTGACTGGGAAAACCCTGGTGCGCCGTCGTGATGCATGACAGTTAGG17620.11394898551774359No Hit
GTCGTGACTGGGAAAACCCTGGGCCTCTGTCGTGATAGACCAACCTTAGG17600.11381964501204808No Hit
GTCGTGACTGGGAAAACCCTGGACCAATGTCGTGATAATCAGTAGTTAGG17550.1134962937478093No Hit
GTCGTGACTGGGAAAACCCTGGTCTGGTGTCGTGATAGGATCAGCTTAGG17530.1133669532421138No Hit
GTCGTGACTGGGAAAACCCTGGTACGACGTCGTGATCTAGGCACCTTAGG17500.11317294248357052No Hit
GTCGTGACTGGGAAAACCCTGGCCCCCCGTCGTGATGCCTACACTTTAGG17460.1129142614721795No Hit
GTCGTGACTGGGAAAACCCTGGTCAGCGGTCGTGATCGAATCACTTTAGG17450.11284959121933176No Hit
GTCGTGACTGGGAAAACCCTGGAGCCCTGTCGTGATGACATGACCTTAGG17380.11239689944939747No Hit
GTCGTGACTGGGAAAACCCTGGGAGAGCGTCGTGATACTGAGGACTTAGG17200.11123283489813789No Hit
GTCGTGACTGGGAAAACCCTGGCGTAAGGTCGTGATACAAATTCCTTAGG17130.1107801431282036No Hit
GTCGTGACTGGGAAAACCCTGGACATCGGTCGTGATCGAATTTATTTAGG17120.11071547287535585No Hit
GTCGTGACTGGGAAAACCCTGGGAGTTCGTCGTGATGTGAGCTGTTTAGG16980.10981008933548728No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGCGTCGTGATGGGAGTAGATTAGG16970.10974541908263953No Hit
GTCGTGACTGGGAAAACCCTGGAACTTAGTCGTGATATCAGTACATTAGG16840.10890470579561873No Hit
GTCGTGACTGGGAAAACCCTGGTAGTCTGTCGTGATTGCAATATGTTAGG16830.10884003554277097No Hit
GTCGTGACTGGGAAAACCCTGGTATTCGTCGTGATGCAATGACGATTAGG16790.10858135453137994No Hit
GTCGTGACTGGGAAAACCCTGGCCTCAGTCGTGATTCAAGGGGCGTTAGG16750.10832267351998894No Hit
GTCGTGACTGGGAAAACCCTGGTTGCATGTCGTGATTAGGAAGACTTAGG16710.10806399250859791No Hit
GTCGTGACTGGGAAAACCCTGGGTAACTGTCGTGATGGCCGTCATTTAGG16640.10761130073866362No Hit
GTCGTGACTGGGAAAACCCTGGTCCTCCGTCGTGATATAGCTACTTTAGG16530.10689992795733833No Hit
GTCGTGACTGGGAAAACCCTGGGTACAAGTCGTGATGCTAAGATATTAGG16510.10677058745164282No Hit
GTCGTGACTGGGAAAACCCTGGTTGCCTGTCGTGATATGGCTCCTTTAGG16480.10657657669309954No Hit
GTCGTGACTGGGAAAACCCTGGACGTAGGTCGTGATAAGAGGGTTTTAGG16440.10631789568170853No Hit
GTCGTGACTGGGAAAACCCTGGAATGAGGTCGTGATCCTTCAGATTTAGG16310.10547718239468773No Hit
GTCGTGACTGGGAAAACCCTGGATTTACGTCGTGATGAGAATTTGTTAGG16230.1049598203719057No Hit
GTCGTGACTGGGAAAACCCTGGTTGGACGTCGTGATCTACAAAGATTAGG16140.1043777880962759No Hit
GTCGTGACTGGGAAAACCCTGGATTCTAGTCGTGATTTACATTGCTTAGG16120.10424844759058038No Hit
GTCGTGACTGGGAAAACCCTGGTTTGAAGTCGTGATTATATACCTTTAGG16040.10373108556779836No Hit
GTCGTGACTGGGAAAACCCTGGCCCAACGTCGTGATATGTATAGGTTAGG16030.10366641531495059No Hit
GTCGTGACTGGGAAAACCCTGGGTACATGTAGCACCAATTTCACCTTAGG16030.10366641531495059No Hit
GTCGTGACTGGGAAAACCCTGGCCCATTGTCGTGATGGATGCGGTTTAGG16020.10360174506210285No Hit
GTCGTGACTGGGAAAACCCTGGATCGTTGTCGTGATCCCATTTCCTTAGG16010.10353707480925509No Hit
GTCGTGACTGGGAAAACCCTGGCAATAAGTCGTGATCCTGAGTGATTAGG16000.10347240455640734No Hit
GTCGTGACTGGGAAAACCCTGGTCAGCCGTCGTGATCGCATCACTTTAGG15990.10340773430355958No Hit
GTCGTGACTGGGAAAACCCTGGAATAATGTCGTGATTCGGACGACTTAGG15920.1029550425336253No Hit
GTCGTGACTGGGAAAACCCTGGGATTCAGTCGTGATCACCCGCCCTTAGG15910.10289037228077755No Hit
GTCGTGACTGGGAAAACCCTGGTTGTCGGTCGTGATGATGAGTAGTTAGG15840.10243768051084326No Hit
GTCGTGACTGGGAAAACCCTGGCGCCAAGTCGTGATGTATATAAATTAGG15810.1022436697523No Hit
GTCGTGACTGGGAAAACCCTGGTTCCAGGTCGTGATGGGGAATATTTAGG15800.10217899949945225No Hit
GTCGTGACTGGGAAAACCCTGGGAATTCGTCGTGATGACTACACTTTAGG15780.10204965899375674No Hit
GTCGTGACTGGGAAAACCCTGGTATTCAGTCGTGATATCGAGACTTTAGG15780.10204965899375674No Hit
GTCGTGACTGGGAAAACCCTGGATTGTTGTCGTGATTTGTACTCTTTAGG15750.10185564823521347No Hit
GTCGTGACTGGGAAAACCCTGGGCAACCGTCGTGATGAACTGAGCTTAGG15740.10179097798236572No Hit
GTCGTGACTGGGAAAACCCTGGATTCCGGTCGTGATCGGTATTATTTAGG15710.10159696722382246No Hit
GTCGTGACTGGGAAAACCCTGGGGCGAAGTCGTGATGGATAATATTTAGG15650.10120894570673593No Hit
GTCGTGACTGGGAAAACCCTGGCCGGGCTGTCGTGATACCCGAGATTAGG15610.10095026469534492No Hit
GTCGTGACTGGGAAAACCCTGGCAGTTGGCGTGATAACAATCTCTTTAGG15580.10075625393680164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC1484500.0240.644132
ACTGGGA1479300.0240.585597
TGGGAAA1419800.0240.201819
GTGACTG1490600.0240.130374
TGACTGG1492550.0239.770485
GACTGGG1499100.0238.553976
CGTGACT1518100.0234.700093
CTGGGAA1540600.0231.672648
TGCGAAA1150.0231.511149
CGTGGCT18050.0222.078743
TCGTTGC700.0207.686422
TCGTGGC20500.0164.882752
CGGACTG1050.0161.471153
GTGACTT8850.0161.41894
GTCGACT402.8587237E-8151.487081
GCGTGGC6950.0139.453722
ACGTGAC1850.0137.522082
TGGGAAC76350.0136.946769
TGGTGAC21750.0134.797242
GTGGTGA22500.0131.423451
Produced by FastQC (version 0.11.5)