FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503289
Sequences flagged as poor quality0
Sequence length248
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGTTTTTTGTCGTGATGTGCGGATGATCAC28930.5748188416595634No Hit
GTCGTGACTGGGAAAACCCTGGCCGAACGTCGTGATATGCGCGGTATCAC15500.30797414606717016No Hit
GTCGTGACTGGGAAAACCCTGGAGCTTGGTCGTGATTGCAAGACGATCAC14380.28572053035134887No Hit
GTCGTGACTGGGAAAACCCTGGACAAATGTCGTGATGGACTGTGGATCAC13260.2634669146355275No Hit
GTCGTGACTGGGAAAACCCTGGTAGGTCGTCGTGATATTAATGGGATCAC13220.26267214264567673No Hit
GTCGTGACTGGGAAAACCCTGGTATCGTGTCGTGATGTGCGTGAGATCAC13030.25889697569388564No Hit
GTCGTGACTGGGAAAACCCTGGGAGAAAGTCGTGATTGAGTGATAATCAC12830.2549231157446318No Hit
GTCGTGACTGGGAAAACCCTGGTCTAGCGTCGTGATAAGGCGAGGATCAC11670.23187472803895973No Hit
GTCGTGACTGGGAAAACCCTGGTTCCACGTCGTGATGATAGCTCTATCAC10720.21299889328000415No Hit
GTCGTGACTGGGAAAACCCTGGACATTGGTCGTGATCGCTTGAACATCAC10390.20644202436373532No Hit
GTCGTGACTGGGAAAACCCTGGGATTTTGTCGTGATGTTCGTGAGATCAC9940.19750083947791428No Hit
GTCGTGACTGGGAAAACCCTGGGAGTCTGTCGTGATGCCCGGGTCATCAC9570.19014919857179474No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTCGTGATGCCGCGAGATCAC9390.1865727246174663No Hit
GTCGTGACTGGGAAAACCCTGGCGCTCTGTCGTGATTAGGTGGGCATCAC9340.18557925963015284No Hit
GTCGTGACTGGGAAAACCCTGGATCGCGGTCGTGATCATGTTGCAATCAC9330.18538056663269017No Hit
GTCGTGACTGGGAAAACCCTGGAGCTGCGTCGTGATATGCCCTAAATCAC9260.18398971565045133No Hit
GTCGTGACTGGGAAAACCCTGGTGTTCAGTCGTGATTGCGATGGCATCAC9170.18220147867328712No Hit
GTCGTGACTGGGAAAACCCTGGACGAAAGTCGTGATGTCCCGGGGATCAC9020.17922108371134676No Hit
GTCGTGACTGGGAAAACCCTGGATATCAGTCGTGATGACAACACAATCAC8790.1746511447697049No Hit
GTCGTGACTGGGAAAACCCTGGGACAAAGTCGTGATGCCTAGCGGATCAC8550.16988251283060032No Hit
GTCGTGACTGGGAAAACCCTGGCGTCACGTCGTGATAGGTATAAAATCAC8530.16948512683567493No Hit
GTCGTGACTGGGAAAACCCTGACCCTGCCGTCGTGATAAAAAAAGATCAC8340.16570995988388382No Hit
GTCGTGACTGGGAAAACCCTGGGATGCCGTCGTGATGTGAATCAGATCAC8280.16451780189910767No Hit
GTCGTGACTGGGAAAACCCTGGATCCATGTCGTGATTTAACCAGGATCAC8230.16352433691179422No Hit
GTCGTGACTGGGAAAACCCTGGACTACGGTCGTGATATACAATACATCAC8170.16233217892701807No Hit
GTCGTGACTGGGAAAACCCTGGCTAGGAGTCGTGATGGGAACCTCATCAC7580.15060929207671936No Hit
GTCGTGACTGGGAAAACCCTGGCTCCTAGTCGTGATAGCTTCATTATCAC7340.14584066013761476No Hit
GTCGTGACTGGGAAAACCCTGGTGGTGCGTCGTGATCGTAAGCTGATCAC7220.1434563441680625No Hit
GTCGTGACTGGGAAAACCCTGGGGTATAGTCGTGATTACTACAAAATCAC7070.1404759492061221No Hit
GTCGTGACTGGGAAAACCCTGGAACGGTGTCGTGATGTCATGGGAATCAC6840.13590601026448024No Hit
GTCGTGACTGGGAAAACCCTGGAGCACTGTCGTGATTTATATGACATCAC6810.13530993127209218No Hit
GTCGTGACTGGGAAAACCCTGGGACTACGTCGTGATTTACTACCCATCAC6650.13213084331268912No Hit
GTCGTGACTGGGAAAACCCTGGATCAAGGTCGTGATTGTCATCAGATCAC6600.13113737832537567No Hit
GTCGTGACTGGGAAAACCCTGGGATATGGTCGTGATGGGTAGTAGATCAC6490.1289517553532861No Hit
GTCGTGACTGGGAAAACCCTGGTTGCTGGTCGTGATAGCACGTAGATCAC6400.12716351837612186No Hit
GTCGTGACTGGGAAAACCCTGGAATAAAGTCGTGATCACCGGGTGATCAC6370.1265674393837338No Hit
GTCGTGACTGGGAAAACCCTGGTTGCTTGTCGTGATGGGTGCGTGATCAC6370.1265674393837338No Hit
GTCGTGACTGGGAAAACCCTGGTGGACCGTCGTGATGTCATGTTCATCAC6090.12100403545477847No Hit
GTCGTGACTGGGAAAACCCTGGGTTCACGTCGTGATTCGAAGTGTATCAC6050.1202092634649277No Hit
GTCGTGACTGGGAAAACCCTGGTTTGCAGTCGTGATTGCAAGTCTATCAC6040.12001057046746502No Hit
GTCGTGACTGGGAAAACCCTGGAAAGTCGTCGTGATCACAATCCTATCAC5980.11881841248268887No Hit
GTCGTGACTGGGAAAACCCTGGGTTTCTGTCGTGATCTCTTCAATATCAC5960.1184210264877635No Hit
GTCGTGACTGGGAAAACCCTGGCTCTGGGTCGTGATCGCCTCTCTATCAC5950.1182223334903008No Hit
GTCGTGACTGGGAAAACCCTGGCCTAAGGTCGTGAATTTATTACTATCAC5850.11623540351567391No Hit
GTCGTGACTGGGAAAACCCTGGTAAAAAGTCGTGATCCCCGGTTGATCAC5830.11583801752074852No Hit
GTCGTGACTGGGAAAACCCTGGTGAGGAGTCGTGATTACCCGTCTATCAC5780.11484455253343506No Hit
GTCGTGACTGGGAAAACCCTGGATTTTCGTCGTGATTATGATCGTATCAC5560.11047330658925586No Hit
GTCGTGACTGGGAAAACCCTGGAACCTGGTCGTGATGAACTAAAAATCAC5510.10947984160194243No Hit
GTCGTGACTGGGAAAACCCTGGACGCGTGTCGTGATAGTAGCAAAATCAC5460.10848637661462898No Hit
GTCGTGACTGGGAAAACCCTGGATTGTCGTCGTGATTAAAAGGTAATCAC5440.10808899061970359No Hit
GTCGTGACTGGGAAAACCCTGGGTACTTGTCGTGATCCATTCACAATCAC5420.10769160462477821No Hit
GTCGTGACTGGGAAAACCCTGGCATTAAGTCGTGATTGGAGCAGCATCAC5280.10490990266030054No Hit
GTCGTGACTGGGAAAACCCTGGAAGTAAGTCGTGATCGGTTGGAGATCAC5260.10451251666537516No Hit
GTCGTGACTGGGAAAACCCTGGTGGTGCGTCGTGATAGTTTACAAATCAC5240.1041151306704498No Hit
GTCGTGACTGGGAAAACCCTGGTGCTTCGTCGTGATGGACATAGCATCAC5180.10292297268567364No Hit
GTCGTGACTGGGAAAACCCTGGGCGGCCGTCGTGATATGACGCCTATCAC5100.10133342870597212No Hit
GTCGTGACTGGGAAAACCCTGGTGTATGGTCGTGATGACTTGTGAATCAC5050.10033996371865865No Hit
GTCGTGACTGGGAAAACCCTGGTCTATAGTCGTGATCTTAACACCATCAC5050.10033996371865865No Hit
GTCGTGACTGGGAAAACCCTGGCAGGTAGTCGTGATATCCTACTAATCAC5040.10014127072119597No Hit
GTCGTGACTGGGAAAACCCTGGTGGCCTGTCGTGATCAATACGCCATCAC5040.10014127072119597No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)