FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507819
Sequences flagged as poor quality0
Sequence length248
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGACTGGGAAAACCCTGGCGCCTGGTCGTGATTGCGAGTTCGATCA51331.0107932156929929No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGATCA11680.23000320980506836No Hit
GTCGTGACTGGGAAAACCCTGGTAATGTGTCGTGATGTTTAGGAAGATCA10670.2101142336147328No Hit
GTCGTGACTGGGAAAACCCTGGTCGAAAGTCGTGATATAGGAGAAGATCA8050.1585210478536644No Hit
GTCGTGACTGGGAAAACCCTGGGTAGCTGTCGTGATGGATTTACAGATCA7770.15300727227614563No Hit
GTCGTGACTGGGAAAACCCTGGAGCGTAGTCGTGATAAAAACATTGATCA7240.14257048279012796No Hit
GTCGTGACTGGGAAAACCCTGGCTCGGGGTCGTGATCCATATCTGGATCA7210.14197972112110813No Hit
GTCGTGACTGGGAAAACCCTGGTGTCTGGTCGTGATCCGTAAGTCGATCA7050.13882899221966882No Hit
GTCGTGACTGGGAAAACCCTGGATTCACGTCGTGATCATAGATCCGATCA6890.13567826331822952No Hit
GTCGTGACTGGGAAAACCCTGGCCTCGTGTCGTGATAAGGATACCGATCA6740.13272445497313018No Hit
GTCGTGACTGGGAAAACCCTGGACCCGTGTCGTGATAGTCGTCGGGATCA6660.13114909052241056No Hit
GTCGTGACTGGGAAAACCCTGGCCCAGTGTCGTGATGTCCGGGATGATCA6430.12661991772659156No Hit
GTCGTGACTGGGAAAACCCTGGGGCTGCGTGTGAATTCCTGGAGTGATCA6200.12209074493077259No Hit
GTCGTGACTGGGAAAACCCTGGGGAGCTGTCGTGATAACAACTTTGATCA6090.11992461881103307No Hit
GTCGTGACTGGGAAAACCCTGGGCATGAGTCGTGATCCCTGGGGTGATCA6000.11815233380397347No Hit
GTCGTGACTGGGAAAACCCTGGCGGGCTGTCGTGATGCCGGCGAGGATCA5930.11677388990959377No Hit
GTCGTGACTGGGAAAACCCTGGCGGTCGGTCGTGATTGGGACGAGGATCA5920.11657696935325382No Hit
GTCGTGACTGGGAAAACCCTGGTTATGAGTCGTGAAGTAGGACAGGATCA5840.11500160490253418No Hit
GTCGTGACTGGGAAAACCCTGGGGCTCGGTCGTGATGGGTAGTTTGATCA5800.11421392267717434No Hit
GTCGTGACTGGGAAAACCCTGGCCTCTGTCGTGATCCTACTGGTCGATCA5770.11362316100815449No Hit
GTCGTGACTGGGAAAACCCTGGGTATGTGTCGTGATTGATGCGAGGATCA5700.11224471711377478No Hit
GTCGTGACTGGGAAAACCCTGGATGAATGTCGTGATAAAGGCAAGGATCA5680.11185087600109488No Hit
GTCGTGACTGGGAAAACCCTGGGCATTCGTCGTGTAGTCTAAAGTGATCA5650.11126011433207501No Hit
GTCGTGACTGGGAAAACCCTGGGATACCGTCGGATCAGGATGGCTGATCA5610.11047243210671519No Hit
GTCGTGACTGGGAAAACCCTGGCGCCCTGTCGTGATAACTAGTGTGATCA5570.10968474988135536No Hit
GTCGTGACTGGGAAAACCCTGGGCAGGAGTCGTGATCAAGGATATGATCA5460.10751862376161585No Hit
GTCGTGACTGGGAAAACCCTGGCTATTGGTCGTGATGAACAGGCTGATCA5380.10594325931089621No Hit
GTCGTGACTGGGAAAACCCTGGCGTCACGTCGTGATACCACCGATGATCA5170.10180792762775714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT497350.0241.722963
TGACTGG498450.0241.24075
TCGTGAC499200.0241.188062
GTGACTG498650.0241.0214
GACTGGG498750.0240.780676
ACTGGGA511800.0234.405277
TGGGAAA512200.0234.151939
CTGGGAA551500.0217.465688
CGTTGCC150.0051407567161.457033
ACCGGGA1400.0129.652837
ACGTGAC1050.0126.934142
TGACCGG1550.0124.937445
GTCGTGA970050.0123.9424741
CTCGTGA1650.0102.846741
CGTGACA1400.095.144333
TTGTGAC903.5106495E-1094.238992
CGTGACC1700.092.6003653
TGCGAAA552.5486519E-587.99819
GACCGGG2350.087.5472646
CGAGACT1250.087.1868063
Produced by FastQC (version 0.11.5)