FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760660
Sequences flagged as poor quality0
Sequence length248
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGATG11900.15644308889648462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGA746400.0240.514347
TCGTGAC750100.0240.176362
TGGGAAA754500.0237.964349
GTGACTG766550.0234.552124
CGTGACT767300.0234.543053
TGACTGG768150.0234.080815
GACTGGG772400.0232.62126
CTGGGAA775650.0231.522458
CTGCGAA900.0188.214058
TGCGAAA1100.0153.99339
CGAGACT1950.0149.014563
TCGAGAC1850.0137.517142
TCGGACT850.0128.272282
GTCGTGA1452200.0124.0317461
CGTTACT501.0884469E-7121.0743263
ACCGGGA2300.0120.9947367
GTCGTAC851.8189894E-12114.049831
GTCGGAC903.6379788E-12107.713741
TCGTTAC801.382432E-10106.0027852
TCGTACT955.456968E-12102.017722
Produced by FastQC (version 0.11.5)