FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022640
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCAGAT14740.14413674411327546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT1002400.0241.194853
GTGACTG1002700.0241.038764
TGACTGG1002750.0240.980445
TCGTGAC1005300.0240.724672
ACTGGGA1002550.0240.46137
GACTGGG1005250.0240.140416
CTGGGAA1030350.0233.808988
TGGGAAA1034700.0232.826029
TGCGAAA1350.0170.283579
GAGATCG1650.0139.322927
CGAGACT1850.0137.401553
ACCGGGA2400.0131.073537
ACGTGAC1600.0128.685032
GTCGTGA1950500.0124.0517041
TCGAGAC2150.0118.298682
CGTTACT758.367351E-11112.97463
CGGGGAA2800.0108.027639
TGACCGG3600.097.4649355
ACTGGGG6150.092.464647
AGATCGG6100.091.2390758
Produced by FastQC (version 0.11.5)