FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4360781
Sequences flagged as poor quality0
Sequence length248
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT4296250.0241.281953
GTGACTG4299350.0241.058554
TCGTGAC4303500.0241.018942
TGACTGG4299700.0241.011935
ACTGGGA4315100.0239.672797
GACTGGG4323050.0239.436176
CTGGGAA4422150.0233.799748
TGGGAAA4525150.0228.478069
ACGTGAC6150.0173.368932
CGAGACT11500.0134.766433
GTCGTGA8380550.0123.731811
TCGAGAC13350.0115.2622
ACCGGGA9300.0114.493397
GACGTGA10450.0104.381751
CGTGGCT15100.089.005263
ACTGGGG17750.087.937077
TGACCGG13850.080.3838045
GAACCAG410450.078.26783240-242
CGAGTTA40200.078.15723240-242
AACCAGG399900.077.9726240-242
Produced by FastQC (version 0.11.5)